8Y0B
Crystal structure of FnCas12a in complex with pre-crRNA and 12nt target DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | RNA (53-MER) | polymer | 58 | 18588.0 | 1 | Francisella tularensis subsp. novicida U112 | ||
| 2 | B (C) | DNA (5'-D(P*AP*AP*AP*TP*GP*AP*CP*TP*TP*CP*TP*CP*TP*AP*AP*AP*GP*GP*AP*CP*T)-3') | polymer | 21 | 6430.2 | 1 | Francisella tularensis subsp. novicida U112 | ||
| 3 | C (D) | DNA (5'-D(P*AP*GP*TP*CP*CP*TP*TP*TP*AP*GP*AP*TP*A)-3') | polymer | 18 | 5529.6 | 1 | Francisella tularensis subsp. novicida U112 | ||
| 4 | D (A) | CRISPR-associated endonuclease Cas12a | polymer | 1300 | 152083.1 | 1 | UniProt (A0Q7Q2) Pfam (PF18501) Pfam (PF21918) Pfam (PF22222) Pfam (PF18516) Pfam (PF18510) | Francisella tularensis subsp. novicida U112 | CRISPR-associated endonuclease Cpf1,FnCas12a,FnCpf1 |
| 5 | E, F (B, A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 6 | G, H, I, J (B, C, D, A) | water | water | 18.0 | 568 | Chemie (HOH) |
Sequence modifications
A: 1 - 1300 (UniProt: A0Q7Q2)
| PDB | External Database | Details |
|---|---|---|
| Ala 1006 | Glu 1006 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 182630.9 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 48.6 | |
| All* | Total formula weight | 182679.5 |
