8Y0B

Crystal structure of FnCas12a in complex with pre-crRNA and 12nt target DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-25
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.98322
Spacegroup name	P 43 21 2
Unit cell lengths	124.729, 124.729, 267.756
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.210 - 2.300
R-factor	0.205
Rwork	0.203
R-free	0.24750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mga
RMSD bond length	0.009
RMSD bond angle	1.084
Data reduction software	HKL-3000 (7.21)
Data scaling software	HKL-3000 (7.21)
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	88.200	2.420
High resolution limit [Å]	2.300	2.300
Rpim	0.044	0.395
Number of reflections	91977	13638
<I/σ(I)>	15.9	2.3
Completeness [%]	97.2	100
Redundancy	13.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.05 M MES, pH 6.0, 0.15 M sodium formate, 15% PEG 3350