8Y0B
Crystal structure of FnCas12a in complex with pre-crRNA and 12nt target DNA
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003677 | molecular_function | DNA binding |
| A | 0003723 | molecular_function | RNA binding |
| A | 0004518 | molecular_function | nuclease activity |
| A | 0004519 | molecular_function | endonuclease activity |
| A | 0004530 | molecular_function | deoxyribonuclease I activity |
| A | 0006259 | biological_process | DNA metabolic process |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0016829 | molecular_function | lyase activity |
| A | 0051607 | biological_process | defense response to virus |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 23 |
| Details | Region: {"description":"Wedge region 1","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000303"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 314 |
| Details | Region: {"description":"Recognition domain 1","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000303"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 8 |
| Details | Region: {"description":"Binds crRNA alone and in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | Region: {"description":"Binds DNA in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 3 |
| Details | Region: {"description":"Binds crRNA in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 3 |
| Details | Region: {"description":"Binds crRNA in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 8 |
| Details | Region: {"description":"Binds crRNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 70 |
| Details | Region: {"description":"Wedge region 2","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000303"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 17 |
| Details | Region: {"description":"LKL, important for PAM recognition and DNA unwinding","evidences":[{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 99 |
| Details | Region: {"description":"PAM-interacting domain (PI)","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000303"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI11 |
| Number of Residues | 6 |
| Details | Region: {"description":"Binds DNA protospacer adjacent motif (PAM) on target DNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI12 |
| Number of Residues | 12 |
| Details | Region: {"description":"Binds single-strand non-target DNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI13 |
| Number of Residues | 129 |
| Details | Region: {"description":"Wedge region 3","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000303"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI14 |
| Number of Residues | 10 |
| Details | Region: {"description":"Binds crRNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI15 |
| Number of Residues | 60 |
| Details | Region: {"description":"RuvC-I","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000303"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI16 |
| Number of Residues | 17 |
| Details | Region: {"description":"Bridge helix","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000303"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI17 |
| Number of Residues | 45 |
| Details | Region: {"description":"RuvC-III","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000303"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI18 |
| Number of Residues | 3 |
| Details | Active site: {"description":"For pre-crRNA processing","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI19 |
| Number of Residues | 3 |
| Details | Active site: {"description":"For DNase activity of RuvC domain","evidences":[{"source":"PubMed","id":"26422227","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI20 |
| Number of Residues | 1 |
| Details | Site: {"description":"Binds crRNA alone and in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI21 |
| Number of Residues | 1 |
| Details | Site: {"description":"Binds target strand DNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI22 |
| Number of Residues | 1 |
| Details | Site: {"description":"Binds crRNA in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI23 |
| Number of Residues | 3 |
| Details | Site: {"description":"Binds DNA in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI24 |
| Number of Residues | 1 |
| Details | Site: {"description":"Caps the crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI25 |
| Number of Residues | 1 |
| Details | Site: {"description":"Binds DNA protospacer adjacent motif (PAM)","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI26 |
| Number of Residues | 1 |
| Details | Site: {"description":"Binds Target strand DNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI27 |
| Number of Residues | 1 |
| Details | Site: {"description":"Binds PAM","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI28 |
| Number of Residues | 2 |
| Details | Site: {"description":"Binds Target strand DNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI29 |
| Number of Residues | 1 |
| Details | Site: {"description":"Binds Target strand DNA; via amide nitrogen","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI30 |
| Number of Residues | 1 |
| Details | Site: {"description":"Binds crRNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI31 |
| Number of Residues | 1 |
| Details | Site: {"description":"Stabilizes transition state for pre-crRNA processing","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI32 |
| Number of Residues | 2 |
| Details | Site: {"description":"Binds DNA in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
