8XWP

Cryo-EM structure of ET-1 bound ETBR-DNGI complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Endothelin receptor type B	polymer	346	38808.3	1	UniProt (P24530) Pfam (PF00001)	Homo sapiens (human)	ET-B,ET-BR,Endothelin receptor non-selective type
2	B (L)	Endothelin-1	polymer	21	2498.0	1	UniProt (P05305) Pfam (PF00322)	Homo sapiens (human)	Preproendothelin-1,PPET1
3	C (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40414.0	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
4	D (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	344	37671.1	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
5	E (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
6	F (D)	SCFV16	polymer	277	29398.9	1		Mus musculus

Sequence modifications

R: 66 - 407 (UniProt: P24530)

PDB	External Database	Details
Pro 62	-	expression tag
Gly 63	-	expression tag
Gly 64	-	expression tag
Gly 65	-	expression tag
Tyr 124	Arg 124	conflict
Ala 396	Cys 396	conflict
Ala 400	Cys 400	conflict
Ala 405	Cys 405	conflict

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	156651.4
All*	Total formula weight	156651.4

*Water molecules are not included.