8WU8

Crystal structure of the human RAD9-RAD1(F64A/M256A/F266A)-HUS1-RHINO(88-99) complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Cell cycle checkpoint control protein RAD9A	polymer	270	29746.4	1	UniProt (Q99638) Pfam (PF04139)	Homo sapiens (human)	hRAD9,DNA repair exonuclease rad9 homolog A
2	B (B)	Checkpoint protein HUS1	polymer	286	32560.7	1	UniProt (O60921) Pfam (PF04005)	Homo sapiens (human)	hHUS1
3	C (C)	Cell cycle checkpoint protein RAD1	polymer	282	31641.9	1	UniProt (O60671) Pfam (PF02144)	Homo sapiens (human)	hRAD1,DNA repair exonuclease rad1 homolog,Rad1-like DNA damage checkpoint protein
4	D (D)	RAD9, HUS1, RAD1-interacting nuclear orphan protein 1	polymer	12	1392.6	1	UniProt (Q9BSD3)	Homo sapiens (human)	RAD9,RAD1,HUS1-interacting nuclear orphan protein

Sequence modifications

B: 2 - 280 (UniProt: O60921)

PDB	External Database	Details
Met -5	-	initiating methionine
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag

C: 1 - 282 (UniProt: O60671)

PDB	External Database	Details
Ala 64	Phe 64	engineered mutation
Ala 256	Met 256	engineered mutation
Ala 266	Phe 266	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	95341.6
All*	Total formula weight	95341.6

*Water molecules are not included.