8WU8

Crystal structure of the human RAD9-RAD1(F64A/M256A/F266A)-HUS1-RHINO(88-99) complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.980
Spacegroup name	P 1 21 1
Unit cell lengths	71.115, 69.938, 83.948
Unit cell angles	90.00, 97.36, 90.00

Refinement procedure

Resolution	35.771 - 2.810
R-factor	0.2202
Rwork	0.215
R-free	0.26690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	0.800
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.771	2.911
High resolution limit [Å]	2.810	2.810
Number of reflections	19831	1984
<I/σ(I)>	15.87
Completeness [%]	98.5
Redundancy	3.4
CC(1/2)	0.997	0.659

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, Potassium iodine