8WSS

Cryo-EM structure of Melanin-Concentrating Hormone Receptor 1 with MCH

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40414.0	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	377	41055.9	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (H)	Scfv16	polymer	285	30363.0	1		Mus musculus
5	E (L)	Pro-MCH	polymer	19	2391.9	1	UniProt (P20382)	Homo sapiens (human)
6	F (R)	Melanin-concentrating hormone receptor 1	polymer	402	43716.8	1	UniProt (Q99705) Pfam (PF00001)	Homo sapiens (human)	MCH receptor 1,MCH-R1,MCHR-1,G-protein coupled receptor 24,MCH-1R,MCH1R,MCHR,SLC-1,Somatostatin receptor-like protein

Sequence modifications

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	initiating methionine
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

R: 70 - 405 (UniProt: Q99705)

PDB	External Database	Details
Gln 66	-	expression tag
Gly 67	-	expression tag
Gly 68	-	expression tag
Thr 69	-	expression tag
Gly 406	-	expression tag
Ser 407	-	expression tag
Ser 408	-	expression tag
Gly 409	-	expression tag
Gly 410	-	expression tag
Gly 411	-	expression tag
Gly 412	-	expression tag
Ser 413	-	expression tag
Gly 414	-	expression tag
Gly 415	-	expression tag
Gly 416	-	expression tag
Gly 417	-	expression tag
Ser 418	-	expression tag
Ser 419	-	expression tag
Gly 420	-	expression tag
Val 421	-	expression tag
Phe 422	-	expression tag
Thr 423	-	expression tag
Leu 424	-	expression tag
Glu 425	-	expression tag
Asp 426	-	expression tag
Phe 427	-	expression tag
Val 428	-	expression tag
Gly 429	-	expression tag
Asp 430	-	expression tag
Trp 431	-	expression tag
Glu 432	-	expression tag
Gln 433	-	expression tag
Thr 434	-	expression tag
Ala 435	-	expression tag
Ala 436	-	expression tag
Tyr 437	-	expression tag
Asn 438	-	expression tag
Leu 439	-	expression tag
Asp 440	-	expression tag
Gln 441	-	expression tag
Val 442	-	expression tag
Leu 443	-	expression tag
Glu 444	-	expression tag
Gln 445	-	expression tag
Gly 446	-	expression tag
Gly 447	-	expression tag
Val 448	-	expression tag
Ser 449	-	expression tag
Ser 450	-	expression tag
Leu 451	-	expression tag
Leu 452	-	expression tag
Gln 453	-	expression tag
Asn 454	-	expression tag
Leu 455	-	expression tag
Ala 456	-	expression tag
Val 457	-	expression tag
Ser 458	-	expression tag
Val 459	-	expression tag
Thr 460	-	expression tag
Pro 461	-	expression tag
Ile 462	-	expression tag
Gln 463	-	expression tag
Arg 464	-	expression tag
Ile 465	-	expression tag
Val 466	-	expression tag
Arg 467	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	165802.7
All*	Total formula weight	165802.7

*Water molecules are not included.