8WQE
Cryo-EM structure of CUL2-RBX1-ELOB-ELOC-FEM1B bound with the C-degron of CUX1 (conformation 1)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, I (C, A) | Cullin-2 | polymer | 750 | 87114.8 | 2 | UniProt (Q13617) Pfam (PF00888) Pfam (PF26557) Pfam (PF10557) | Homo sapiens (human) | CUL-2 |
| 2 | B, J (D, B) | Protein fem-1 homolog B | polymer | 627 | 70355.1 | 2 | UniProt (Q9UK73) Pfam (PF12796) | Homo sapiens (human) | FEM1b,FEM1-beta,Fem-1-like death receptor-binding protein alpha,Fem-1-like in apoptotic pathway protein alpha,F1A-alpha |
| 3 | C, F (E, G) | Elongin-C | polymer | 96 | 10843.4 | 2 | UniProt (Q15369) Pfam (PF03931) | Homo sapiens (human) | EloC,Elongin 15 kDa subunit,RNA polymerase II transcription factor SIII subunit C,SIII p15,Transcription elongation factor B polypeptide 1 |
| 4 | D, G (F, H) | Elongin-B | polymer | 118 | 13147.8 | 2 | UniProt (Q15370) Pfam (PF00240) | Homo sapiens (human) | EloB,Elongin 18 kDa subunit,RNA polymerase II transcription factor SIII subunit B,SIII p18,Transcription elongation factor B polypeptide 2 |
| 5 | E, H (J, I) | E3 ubiquitin-protein ligase RBX1, N-terminally processed | polymer | 96 | 11196.8 | 2 | UniProt (P62877) Pfam (PF12678) | Homo sapiens (human) | E3 ubiquitin-protein transferase RBX1,N-terminally processed |
| 6 | K, L (K, L) | Protein CASP | polymer | 31 | 3143.3 | 2 | UniProt (Q13948) | Homo sapiens (human) | |
| 7 | M, N, O, P, Q... (J, I) | ZINC ION | non-polymer | 65.4 | 6 | Chemie (ZN) |
Sequence modifications
C, A: 2 - 745 (UniProt: Q13617)
J, I: 16 - 108 (UniProt: P62877)
K, L: -23 - -1 (UniProt: Q13948)
| PDB | External Database | Details |
|---|---|---|
| Ser -19 | - | expression tag |
| Ala -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Trp -16 | - | expression tag |
| Ser -15 | - | expression tag |
| His -14 | - | expression tag |
| Pro -13 | - | expression tag |
| Gln -12 | - | expression tag |
| Phe -11 | - | expression tag |
| Glu -10 | - | expression tag |
| Lys -9 | - | expression tag |
| Gly -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Gly -6 | - | expression tag |
| Ser -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Thr 1 | - | expression tag |
| - | Lys 117 | deletion |
| - | Leu 118 | deletion |
| - | Thr 119 | deletion |
| - | Glu 120 | deletion |
| - | Ala 121 | deletion |
| - | Asp 122 | deletion |
| - | Leu 123 | deletion |
| - | Gln 124 | deletion |
| - | Tyr 125 | deletion |
| - | Gly 126 | deletion |
| - | Tyr 127 | deletion |
| - | Gly 128 | deletion |
| - | Gly 129 | deletion |
| - | Val 130 | deletion |
| - | Asp 131 | deletion |
| Gly 132 | Met 132 | conflict |
| Gly 133 | Asn 133 | conflict |
| Gly 134 | Glu 134 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ser 13 | - | expression tag |
| His 14 | - | expression tag |
| Met 15 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -31 | - | expression tag |
| Gly -30 | - | expression tag |
| Gly -29 | - | expression tag |
| Ser -28 | - | expression tag |
| Gly -27 | - | expression tag |
| Gly -26 | - | expression tag |
| Gly -25 | - | expression tag |
| Ser -24 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 391602.2 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 392.5 | |
| All* | Total formula weight | 391994.7 |
