8WJX

ADP-bound purinergic receptor 1 in complex with miniGs/q

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	GNAS complex locus	polymer	265	30840.8	1	Pfam (PF00503) UniProt (by SIFTS) (P63092)	Homo sapiens (human)
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	377	41055.9	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (N)	Nanobody 35	polymer	157	17057.3	1		Homo sapiens (human)
5	E (R)	Soluble cytochrome b562,P2Y purinoceptor 1	polymer	680	76586.2	1	UniProt (P0ABE7) UniProt (P47900) Pfam (PF07361) Pfam (PF00001) UniProt (by SIFTS) (Q9GV45)	Escherichia coli	Cytochrome b-562,P2Y1,ADP receptor,Purinergic receptor
6	F (R)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
7	G (R)	CHOLESTEROL	non-polymer		386.7	1	Chemie (CLR)

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	initiating methionine
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

R: -114 - -10 (UniProt: P0ABE7)

PDB	External Database	Details
Met -161	-	initiating methionine
Lys -160	-	expression tag
Thr -159	-	expression tag
Ile -158	-	expression tag
Ile -157	-	expression tag
Ala -156	-	expression tag
Leu -155	-	expression tag
Ser -154	-	expression tag
Tyr -153	-	expression tag
Ile -152	-	expression tag
Phe -151	-	expression tag
Cys -150	-	expression tag
Leu -149	-	expression tag
Val -148	-	expression tag
Phe -147	-	expression tag
Ala -146	-	expression tag
Asp -145	-	expression tag
Tyr -144	-	expression tag
Lys -143	-	expression tag
Asp -142	-	expression tag
Asp -141	-	expression tag
Asp -140	-	expression tag
Asp -139	-	expression tag
Ala -138	-	expression tag
Gly -137	-	expression tag
Arg -136	-	expression tag
Ala -135	-	expression tag
His -134	-	expression tag
His -133	-	expression tag
His -132	-	expression tag
His -131	-	expression tag
His -130	-	expression tag
His -129	-	expression tag
His -128	-	expression tag
His -127	-	expression tag
His -126	-	expression tag
His -125	-	expression tag
Glu -124	-	expression tag
Asn -123	-	expression tag
Leu -122	-	expression tag
Tyr -121	-	expression tag
Phe -120	-	expression tag
Gln -119	-	expression tag
Ser -118	-	expression tag
Gly -117	-	expression tag
Ala -116	-	expression tag
Pro -115	-	expression tag
Trp -108	Met 29	conflict
Ile -13	His 124	conflict
Leu -9	-	linker
Glu -8	-	linker
Phe -7	-	linker
Leu -6	-	linker
Glu -5	-	linker
Val -4	-	linker
Leu -3	-	linker
Phe -2	-	linker
Gln -1	-	linker
Gly 0	-	linker
Pro 1	-	linker

R: 2 - 360 (UniProt: P47900)
R: 361 - 518 (PDB: 8WJX)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	173401.3
Non-Polymers*	Number of molecules	2
	Total formula weight	813.9
All*	Total formula weight	174215.2

*Water molecules are not included.