8W2O
Yeast U1 snRNP with humanized U1C Zinc-Finger domain
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | U1 small nuclear ribonucleoprotein 70 kDa homolog | polymer | 300 | 34506.1 | 1 | UniProt (Q00916) Pfam (PF12220) Pfam (PF00076) | Saccharomyces cerevisiae S288C | U1-70K,U1 small nuclear ribonucleoprotein SNP1,U1 snRNP protein SNP1 |
| 2 | B (B) | U1 small nuclear ribonucleoprotein C | polymer | 231 | 27084.9 | 1 | UniProt (Q05900) Pfam (PF06220) | Saccharomyces cerevisiae S288C | U1 snRNP C,U1-C,U1C |
| 3 | C (C) | U1 small nuclear ribonucleoprotein A | polymer | 350 | 40414.0 | 1 | UniProt (P32605) Pfam (PF00076) | Saccharomyces cerevisiae S288C | U1A,Mutant U1 die protein 1 |
| 4 | D (D) | U1 small nuclear ribonucleoprotein component PRP42 | polymer | 544 | 65222.0 | 1 | UniProt (Q03776) | Saccharomyces cerevisiae S288C | U1 snRNP protein PRP42,65 kDa snRNP protein,Pre-mRNA-processing factor 42 |
| 5 | E (E) | Pre-mRNA-processing factor 39 | polymer | 629 | 74834.7 | 1 | UniProt (P39682) | Saccharomyces cerevisiae S288C | |
| 6 | F (F) | Protein NAM8 | polymer | 523 | 57010.8 | 1 | UniProt (Q00539) Pfam (PF00076) | Saccharomyces cerevisiae S288C | |
| 7 | G (G) | 56 kDa U1 small nuclear ribonucleoprotein component | polymer | 492 | 56575.3 | 1 | UniProt (Q03782) Pfam (PF19097) | Saccharomyces cerevisiae S288C | |
| 8 | H (H) | U1 small nuclear ribonucleoprotein component SNU71 | polymer | 52 | 5974.8 | 1 | UniProt (P53207) | Saccharomyces cerevisiae S288C | |
| 9 | I (I) | Protein LUC7 | polymer | 261 | 30245.9 | 1 | UniProt (Q07508) Pfam (PF03194) | Saccharomyces cerevisiae S288C | |
| 10 | J (K) | Small nuclear ribonucleoprotein-associated protein B | polymer | 196 | 22427.0 | 1 | UniProt (P40018) Pfam (PF01423) | Saccharomyces cerevisiae S288C | snRNP-B,Sm protein B,SmB |
| 11 | K (L) | Small nuclear ribonucleoprotein Sm D1 | polymer | 146 | 16296.8 | 1 | UniProt (Q02260) Pfam (PF01423) | Saccharomyces cerevisiae S288C | Sm-D1,snRNP core protein D1 |
| 12 | L (M) | Small nuclear ribonucleoprotein Sm D2 | polymer | 110 | 12876.1 | 1 | UniProt (Q06217) Pfam (PF01423) | Saccharomyces cerevisiae S288C | Sm-D2,snRNP core protein D2 |
| 13 | M (N) | Small nuclear ribonucleoprotein Sm D3 | polymer | 101 | 11240.1 | 1 | UniProt (P43321) Pfam (PF01423) | Saccharomyces cerevisiae S288C | Sm-D3,snRNP core protein D3 |
| 14 | N (O) | Small nuclear ribonucleoprotein E | polymer | 94 | 10385.1 | 1 | UniProt (Q12330) Pfam (PF01423) | Saccharomyces cerevisiae S288C | snRNP-E,Sm protein E,SmE |
| 15 | O (P) | Small nuclear ribonucleoprotein F | polymer | 86 | 9669.9 | 1 | UniProt (P54999) Pfam (PF01423) | Saccharomyces cerevisiae S288C | snRNP-F,Sm protein F,SmF |
| 16 | P (Q) | Small nuclear ribonucleoprotein G | polymer | 77 | 8490.8 | 1 | UniProt (P40204) Pfam (PF01423) | Saccharomyces cerevisiae S288C | snRNP-G,Sm protein G,SmG |
| 17 | Q (r) | ACT1 pre-mRNA | polymer | 187 | 59778.2 | 1 | Saccharomyces cerevisiae S288C | ||
| 18 | R (R) | U1 snRNA | polymer | 568 | 182114.5 | 1 | Saccharomyces cerevisiae S288C |
Sequence modifications
B: 1 - 231 (UniProt: Q05900)
C: 1 - 298 (UniProt: P32605)
| PDB | External Database | Details |
|---|---|---|
| Pro 2 | Thr 2 | engineered mutation |
| Lys 3 | Arg 3 | engineered mutation |
| Phe 4 | Tyr 4 | engineered mutation |
| Asp 7 | Glu 7 | engineered mutation |
| Asp 10 | His 10 | engineered mutation |
| Thr 11 | Ser 11 | engineered mutation |
| Ser 17 | Thr 17 | engineered mutation |
| Pro 18 | Leu 18 | engineered mutation |
| Thr 23 | Ser 23 | engineered mutation |
| Cys 25 | Leu 25 | engineered mutation |
| Ser 26 | Val 26 | engineered mutation |
| Arg 28 | Lys 28 | engineered mutation |
| Lys 29 | Asn 29 | engineered mutation |
| Lys 31 | Leu 31 | engineered mutation |
| Glu 32 | Arg 32 | engineered mutation |
| Asn 33 | Ile 33 | engineered mutation |
| Val 34 | Thr 34 | engineered mutation |
| Lys 35 | Ala 35 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 299 | - | expression tag |
| Arg 300 | - | expression tag |
| Arg 301 | - | expression tag |
| Ile 302 | - | expression tag |
| Pro 303 | - | expression tag |
| Gly 304 | - | expression tag |
| Leu 305 | - | expression tag |
| Ile 306 | - | expression tag |
| Asn 307 | - | expression tag |
| Pro 308 | - | expression tag |
| Trp 309 | - | expression tag |
| Lys 310 | - | expression tag |
| Arg 311 | - | expression tag |
| Arg 312 | - | expression tag |
| Trp 313 | - | expression tag |
| Lys 314 | - | expression tag |
| Lys 315 | - | expression tag |
| Asn 316 | - | expression tag |
| Phe 317 | - | expression tag |
| Ile 318 | - | expression tag |
| Ala 319 | - | expression tag |
| Val 320 | - | expression tag |
| Ser 321 | - | expression tag |
| Ala 322 | - | expression tag |
| Ala 323 | - | expression tag |
| Asn 324 | - | expression tag |
| Arg 325 | - | expression tag |
| Phe 326 | - | expression tag |
| Lys 327 | - | expression tag |
| Lys 328 | - | expression tag |
| Ile 329 | - | expression tag |
| Ser 330 | - | expression tag |
| Ser 331 | - | expression tag |
| Ser 332 | - | expression tag |
| Gly 333 | - | expression tag |
| Ala 334 | - | expression tag |
| Leu 335 | - | expression tag |
| Asp 336 | - | expression tag |
| Tyr 337 | - | expression tag |
| Asp 338 | - | expression tag |
| Ile 339 | - | expression tag |
| Pro 340 | - | expression tag |
| Thr 341 | - | expression tag |
| Thr 342 | - | expression tag |
| Ala 343 | - | expression tag |
| Ser 344 | - | expression tag |
| Glu 345 | - | expression tag |
| Asn 346 | - | expression tag |
| Leu 347 | - | expression tag |
| Tyr 348 | - | expression tag |
| Phe 349 | - | expression tag |
| Gln 350 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 18 |
| Total formula weight | 725147.1 | |
| All* | Total formula weight | 725147.1 |
