8VSU

Cryo-EM structure of LKB1-STRADalpha-MO25alpha heterocomplex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Calcium-binding protein 39	polymer	362	41997.1	1	UniProt (Q9Y376) Pfam (PF08569) In PDB	Homo sapiens (human)	MO25alpha,Protein Mo25
2	C	Serine/threonine-protein kinase STK11	polymer	449	50649.8	1	UniProt (Q15831) Pfam (PF00069) In PDB	Homo sapiens (human)	Liver kinase B1,LKB1,hLKB1,Renal carcinoma antigen NY-REN-19
3	B	Isoform 3 of STE20-related kinase adapter protein alpha	polymer	429	47861.9	1	UniProt (Q7RTN6) Pfam (PF00069) In PDB	Homo sapiens (human)	STRAD alpha,STE20-related adapter protein,Serologically defined breast cancer antigen NY-BR-96
4	B	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)

Sequence modifications

A: 1 - 341 (UniProt: Q9Y376)

PDB	External Database	Details
Gly 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Ala 345	-	expression tag
Thr 346	-	expression tag
Asn 347	-	expression tag
Phe 348	-	expression tag
Ser 349	-	expression tag
Leu 350	-	expression tag
Leu 351	-	expression tag
Lys 352	-	expression tag
Gln 353	-	expression tag
Ala 354	-	expression tag
Gly 355	-	expression tag
Asp 356	-	expression tag
Val 357	-	expression tag
Glu 358	-	expression tag
Glu 359	-	expression tag
Asn 360	-	expression tag
Pro 361	-	expression tag
Gly 362	-	expression tag

C: 1 - 433 (UniProt: Q15831)

PDB	External Database	Details
Pro -15	-	expression tag
Asp -14	-	expression tag
Tyr -13	-	expression tag
Lys -12	-	expression tag
Asp -11	-	expression tag
Asp -10	-	expression tag
Asp -9	-	expression tag
Asp -8	-	expression tag
Lys -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag

B: 38 - 431 (UniProt: Q7RTN6)

PDB	External Database	Details
Pro 24	-	expression tag
His 25	-	expression tag
His 26	-	expression tag
His 27	-	expression tag
His 28	-	expression tag
His 29	-	expression tag
His 30	-	expression tag
Glu 31	-	expression tag
Asn 32	-	expression tag
Leu 33	-	expression tag
Tyr 34	-	expression tag
Phe 35	-	expression tag
Gln 36	-	expression tag
Gly 37	-	expression tag
Gly 432	-	expression tag
Ser 433	-	expression tag
Gly 434	-	expression tag
Ala 435	-	expression tag
Thr 436	-	expression tag
Asn 437	-	expression tag
Phe 438	-	expression tag
Ser 439	-	expression tag
Leu 440	-	expression tag
Leu 441	-	expression tag
Lys 442	-	expression tag
Gln 443	-	expression tag
Ala 444	-	expression tag
Gly 445	-	expression tag
Asp 446	-	expression tag
Val 447	-	expression tag
Glu 448	-	expression tag
Glu 449	-	expression tag
Asn 450	-	expression tag
Pro 451	-	expression tag
Gly 452	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	140508.8
Non-Polymers*	Number of molecules	1
	Total formula weight	427.2
All*	Total formula weight	140936.0

*Water molecules are not included.