8VMF

Crystal structure of a transition-state mimic of the GSK-3/Axin complex bound to a beta-catenin S45D peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Glycogen synthase kinase-3 beta	polymer	364	41538.7	1	UniProt (Q9WV60) Pfam (PF00069)	Mus musculus (house mouse)	GSK-3 beta,Serine/threonine-protein kinase GSK3B
2	B (B)	Axin-1	polymer	24	2738.1	1	UniProt (O35625) UniProt (by SIFTS) (O15169)	Homo sapiens (human)	Axis inhibition protein 1,Protein Fused
3	C (C)	Catenin beta-1	polymer	28	2890.0	1	UniProt (P35222)	Homo sapiens (human)	Beta-catenin
4	D, E (A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	F (A)	ALUMINUM FLUORIDE	non-polymer		84.0	1	Chemie (AF3)
6	G (A)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
7	H, I, J (A)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
8	K (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
9	L, M (A, B)	water	water		18.0	131	Chemie (HOH)

Sequence modifications

A: 26 - 383 (UniProt: Q9WV60)

PDB	External Database	Details
His 384	-	expression tag
His 385	-	expression tag
His 386	-	expression tag
His 387	-	expression tag
His 388	-	expression tag
His 389	-	expression tag

B: 383 - 402 (UniProt: O35625)

PDB	External Database	Details
Gly 379	-	expression tag
Gly 380	-	expression tag
Ile 381	-	expression tag
Leu 382	-	expression tag

C: 35 - 61 (UniProt: P35222)

PDB	External Database	Details
Met 34	-	initiating methionine
Asp 45	Ser 45	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	47166.8
Non-Polymers*	Number of molecules	8
	Total formula weight	871.5
All*	Total formula weight	48038.4

*Water molecules are not included.