8VHU

Crystal structure of dATP bound E. coli class Ia ribonucleotide reductase alpha construct fused with the C-terminal tail of E. coli class Ia beta subunit

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Fusion protein of Ribonucleoside-diphosphate reductase 1 subunits alpha and beta	polymer	807	90940.4	2	UniProt (P00452) UniProt (P69924) Pfam (PF03477) Pfam (PF00317) Pfam (PF02867) In PDB	Escherichia coli K-12
2	A, B	2'-DEOXYADENOSINE 5'-TRIPHOSPHATE	non-polymer		491.2	3	Chemie (DTP)
3	A, B	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
4	A	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
5	A, B	CHLORIDE ION	non-polymer		35.5	6	Chemie (CL)
6	B	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
7		water	water		18.0	1357	Chemie (HOH)

Sequence modifications

A, B: 1 - 734 (UniProt: P00452)

PDB	External Database	Details
Met -18	-	initiating methionine
Gly -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Leu -5	-	expression tag
Val -4	-	expression tag
Pro -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Arg 735	-	linker
Asp 736	-	linker
Gly 737	-	linker
Ala 1325	-	linker
Glu 1326	-	linker
Asp 1327	-	linker
Ala 1328	-	linker
Gln 1329	-	linker
Asp 1330	-	linker
Asp 1331	-	linker
Leu 1332	-	linker
Val 1333	-	linker
Pro 1334	-	linker
Ser 1335	-	linker
Ile 1336	-	linker
Gln 1337	-	linker
Asp 1338	-	linker
Asp 1339	-	linker
Gly 1340	-	linker

A, B: 1341 - 1375 (UniProt: P69924)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	181880.7
Non-Polymers*	Number of molecules	14
	Total formula weight	1874.3
All*	Total formula weight	183755.0

*Water molecules are not included.