8V6V

Cryo-EM structure of doubly-bound SNF2h-nucleosome complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3.2	polymer	135	15271.9	2	UniProt (P84233) Pfam (PF00125)	Xenopus laevis (African clawed frog)
2	B, F (B, F)	Histone H4	polymer	102	11263.2	2	UniProt (P62799) Pfam (PF15511)	Xenopus laevis (African clawed frog)
3	C, G (C, G)	Histone H2A type 1	polymer	129	13978.2	2	UniProt (P06897) Pfam (PF00125) Pfam (PF16211)	Xenopus laevis (African clawed frog)
4	D, H (D, H)	Histone H2B	polymer	122	13524.8	2	UniProt (P02281) Pfam (PF00125)	Xenopus laevis (African clawed frog)	H2B1.1
5	I (I)	Widom 601 DNA (147-mer) with 60 base pairs flanking DNA (reverse strand)	polymer	207	64200.9	1		synthetic construct
6	J (J)	Widom 601 DNA (147-mer) with 60 base pairs flanking DNA (forward strand)	polymer	207	63626.5	1		synthetic construct
7	K, L (W, X)	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5	polymer	1052	122089.3	2	UniProt (O60264) Pfam (PF00176) Pfam (PF00271) Pfam (PF09110) Pfam (PF09111)	Homo sapiens (human)
8	M, O (W, X)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)
9	N, P (W, X)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

A, E: 1 - 135 (UniProt: P84233)

PDB	External Database	Details
Ala 102	Gly 103	engineered mutation
Ala 110	Cys 111	engineered mutation

C, G: 1 - 129 (UniProt: P06897)

PDB	External Database	Details
Arg 99	Gly 100	engineered mutation
Ser 123	Ala 124	engineered mutation

D, H: 1 - 122 (UniProt: P02281)

PDB	External Database	Details
Thr 29	Ser 33	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	480082.2
Non-Polymers*	Number of molecules	4
	Total formula weight	903.0
All*	Total formula weight	480985.2

*Water molecules are not included.