8UW8

Site-one protease and SPRING

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Membrane-bound transcription factor site-1 protease	polymer	1026	115048.2	1	UniProt (Q14703) Pfam (PF23001) Pfam (PF00082) In PDB	Homo sapiens (human)
2	B	SREBP regulating gene protein	polymer	201	23200.3	1	UniProt (Q9H741) Pfam (PF10218) In PDB	Homo sapiens (human)	SREBF pathway regulator in Golgi 1
3	C	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
4	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
5	A	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
6		water	water		18.0	75	Chemie (HOH)

Sequence modifications

A: 1 - 998 (UniProt: Q14703)

PDB	External Database	Details
Asp 999	-	expression tag
Tyr 1000	-	expression tag
Lys 1001	-	expression tag
Asp 1002	-	expression tag
Asp 1003	-	expression tag
Asp 1004	-	expression tag
Asp 1005	-	expression tag
Lys 1006	-	expression tag
Gly 1007	-	expression tag
Ser 1008	-	expression tag
Asp 1009	-	expression tag
Tyr 1010	-	expression tag
Lys 1011	-	expression tag
Asp 1012	-	expression tag
Asp 1013	-	expression tag
Asp 1014	-	expression tag
Asp 1015	-	expression tag
Lys 1016	-	expression tag
Gly 1017	-	expression tag
Ser 1018	-	expression tag
Asp 1019	-	expression tag
Tyr 1020	-	expression tag
Lys 1021	-	expression tag
Asp 1022	-	expression tag
Asp 1023	-	expression tag
Asp 1024	-	expression tag
Asp 1025	-	expression tag
Lys 1026	-	expression tag

B: 36 - 205 (UniProt: Q9H741)

PDB	External Database	Details
Met 15	-	initiating methionine
Glu 16	-	expression tag
Thr 17	-	expression tag
Asp 18	-	expression tag
Thr 19	-	expression tag
Leu 20	-	expression tag
Leu 21	-	expression tag
Leu 22	-	expression tag
Trp 23	-	expression tag
Val 24	-	expression tag
Leu 25	-	expression tag
Leu 26	-	expression tag
Leu 27	-	expression tag
Trp 28	-	expression tag
Val 29	-	expression tag
Pro 30	-	expression tag
Gly 31	-	expression tag
Ser 32	-	expression tag
Thr 33	-	expression tag
Gly 34	-	expression tag
Asp 35	-	expression tag
His 206	-	expression tag
His 207	-	expression tag
His 208	-	expression tag
His 209	-	expression tag
His 210	-	expression tag
His 211	-	expression tag
His 212	-	expression tag
His 213	-	expression tag
His 214	-	expression tag
His 215	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	138248.5
Branched	Number of molecules	1
	Total formula weight	586.5
Non-Polymers*	Number of molecules	2
	Total formula weight	261.3
All*	Total formula weight	139096.3

*Water molecules are not included.