8TQ1
HIV-1 BG505 Env SOSIP in complex with bovine Fab Bess4 and non-human primate Fab RM20A3
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E, I (A, J, E) | HIV-1 Envelope Glycoprotein BG505 SOSIP.664 gp120 | polymer | 516 | 57946.0 | 3 | UniProt (Q2N0S6) | Human immunodeficiency virus 1 | |
| 2 | B, F, J (B, K, F) | Transmembrane protein gp41 | polymer | 153 | 17146.5 | 3 | UniProt (Q2N0S5) | Human immunodeficiency virus 1 | Env polyprotein |
| 3 | C, G, K (C, M, G) | NHP RM20A3 Fab heavy chain | polymer | 125 | 13511.1 | 3 | Macaca mulatta | ||
| 4 | D, H, L (D, N, I) | NHP RM20A3 Fab light chain | polymer | 128 | 13508.8 | 3 | Macaca mulatta | ||
| 5 | M (H) | Bovine Bess4 Fab heavy chain | polymer | 163 | 17435.3 | 1 | Bos taurus | ||
| 6 | N, O, P, R, S... (L, O, P, R, S...) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 8 | In PDB GlyTouCan (G42666HT) | |||
| 7 | Q, U (Q, U) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 2 | In PDB GlyTouCan (G22768VO) | |||
| 8 | V, X (V, X) | alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 748.7 | 2 | In PDB GlyTouCan (G81315DD) | |||
| 9 | AA, AB, BA, BB, CA... (A, J, K, E, B...) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 45 | Chemie (NAG) |
Sequence modifications
A, J, E: 31 - 508 (UniProt: Q2N0S6)
B, K, F: 512 - 664 (UniProt: Q2N0S5)
| PDB | External Database | Details |
|---|---|---|
| Met -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Met -1 | - | expression tag |
| Lys 0 | - | expression tag |
| Arg 1 | - | expression tag |
| Gly 2 | - | expression tag |
| Leu 3 | - | expression tag |
| Cys 4 | - | expression tag |
| Cys 5 | - | expression tag |
| Val 6 | - | expression tag |
| Leu 7 | - | expression tag |
| Leu 8 | - | expression tag |
| Leu 9 | - | expression tag |
| Cys 10 | - | expression tag |
| Gly 11 | - | expression tag |
| Ala 12 | - | expression tag |
| Val 13 | - | expression tag |
| Phe 14 | - | expression tag |
| Val 15 | - | expression tag |
| Ser 16 | - | expression tag |
| Pro 17 | - | expression tag |
| Ser 18 | - | expression tag |
| Gln 19 | - | expression tag |
| Glu 20 | - | expression tag |
| Ile 21 | - | expression tag |
| His 22 | - | expression tag |
| Ala 23 | - | expression tag |
| Arg 24 | - | expression tag |
| Phe 25 | - | expression tag |
| Arg 26 | - | expression tag |
| Arg 27 | - | expression tag |
| Gly 28 | - | expression tag |
| Ala 29 | - | expression tag |
| Arg 30 | - | expression tag |
| Asn 332 | Thr 330 | engineered mutation |
| Cys 501 | Ala 498 | engineered mutation |
| Arg 509 | - | expression tag |
| Arg 510 | - | expression tag |
| Arg 511 | - | expression tag |
| Arg 512 | - | expression tag |
| Arg 513 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Pro 559 | Ile 556 | conflict |
| Cys 605 | Thr 602 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 13 |
| Total formula weight | 323772.4 | |
| Branched | Number of molecules | 12 |
| Total formula weight | 6714.2 | |
| Non-Polymers* | Number of molecules | 45 |
| Total formula weight | 9954.4 | |
| All* | Total formula weight | 340441.0 |
