8TQ1

HIV-1 BG505 Env SOSIP in complex with bovine Fab Bess4 and non-human primate Fab RM20A3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, J, E	HIV-1 Envelope Glycoprotein BG505 SOSIP.664 gp120	polymer	516	57946.0	3	UniProt (Q2N0S6) In PDB	Human immunodeficiency virus 1
2	B, K, F	Transmembrane protein gp41	polymer	153	17146.5	3	UniProt (Q2N0S5) In PDB	Human immunodeficiency virus 1	Env polyprotein
3	C, M, G	NHP RM20A3 Fab heavy chain	polymer	125	13511.1	3		Macaca mulatta
4	D, N, I	NHP RM20A3 Fab light chain	polymer	128	13508.8	3		Macaca mulatta
5	H	Bovine Bess4 Fab heavy chain	polymer	163	17435.3	1		Bos taurus
6	L, O, P, R, S...	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	8	In PDB GlyTouCan (G42666HT)
7	Q, U	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	2	In PDB GlyTouCan (G22768VO)
8	V, X	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	2	In PDB GlyTouCan (G81315DD)
9	A, J, K, E, B...	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	45	Chemie (NAG)

Sequence modifications

A, J, E: 31 - 508 (UniProt: Q2N0S6)

PDB	External Database	Details
Met -4	-	expression tag
Asp -3	-	expression tag
Ala -2	-	expression tag
Met -1	-	expression tag
Lys 0	-	expression tag
Arg 1	-	expression tag
Gly 2	-	expression tag
Leu 3	-	expression tag
Cys 4	-	expression tag
Cys 5	-	expression tag
Val 6	-	expression tag
Leu 7	-	expression tag
Leu 8	-	expression tag
Leu 9	-	expression tag
Cys 10	-	expression tag
Gly 11	-	expression tag
Ala 12	-	expression tag
Val 13	-	expression tag
Phe 14	-	expression tag
Val 15	-	expression tag
Ser 16	-	expression tag
Pro 17	-	expression tag
Ser 18	-	expression tag
Gln 19	-	expression tag
Glu 20	-	expression tag
Ile 21	-	expression tag
His 22	-	expression tag
Ala 23	-	expression tag
Arg 24	-	expression tag
Phe 25	-	expression tag
Arg 26	-	expression tag
Arg 27	-	expression tag
Gly 28	-	expression tag
Ala 29	-	expression tag
Arg 30	-	expression tag
Asn 332	Thr 330	engineered mutation
Cys 501	Ala 498	engineered mutation
Arg 509	-	expression tag
Arg 510	-	expression tag
Arg 511	-	expression tag
Arg 512	-	expression tag
Arg 513	-	expression tag

B, K, F: 512 - 664 (UniProt: Q2N0S5)

PDB	External Database	Details
Pro 559	Ile 556	conflict
Cys 605	Thr 602	conflict

Contents of the asymmetric unit

Polymers	Number of chains	13
	Total formula weight	323772.4
Branched	Number of molecules	12
	Total formula weight	6714.2
Non-Polymers*	Number of molecules	45
	Total formula weight	9954.4
All*	Total formula weight	340441.0

*Water molecules are not included.