8TLT
Cryo-EM structure of Rev1(deltaN)-Polzeta-DNA-dCTP complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA polymerase zeta catalytic subunit | polymer | 1538 | 177068.5 | 1 | UniProt (P14284) Pfam (PF03104) Pfam (PF00136) Pfam (PF14260) | Saccharomyces cerevisiae (brewer's yeast) | Protein reversionless 3 |
| 2 | B, C (D, E) | DNA polymerase zeta processivity subunit | polymer | 245 | 28791.7 | 2 | UniProt (P38927) Pfam (PF02301) | Saccharomyces cerevisiae (brewer's yeast) | Revertibility protein 7 |
| 3 | D (F) | DNA polymerase delta small subunit | polymer | 494 | 55987.4 | 1 | UniProt (A0A6A5PTG9) Pfam (PF04042) Pfam (PF18018) UniProt (by SIFTS) (P46957) | Saccharomyces cerevisiae (brewer's yeast) | SX2_G0007820.mRNA.1.CDS.1 |
| 4 | E (G) | DNA polymerase delta subunit 3 | polymer | 350 | 40377.7 | 1 | UniProt (P47110) | Saccharomyces cerevisiae (brewer's yeast) | |
| 5 | F (B) | DNA repair protein REV1 | polymer | 985 | 112384.2 | 1 | UniProt (P12689) Pfam (PF16589) Pfam (PF00817) Pfam (PF21999) Pfam (PF11799) | Saccharomyces cerevisiae (brewer's yeast) | Reversionless protein 1 |
| 6 | G (P) | DNA (5'-D(P*CP*CP*CP*TP*CP*CP*CP*CP*TP*AP*C)-3') | polymer | 11 | 3190.1 | 1 | synthetic construct | ||
| 7 | H (T) | DNA (5'-D(*TP*AP*AP*TP*GP*GP*TP*AP*GP*GP*GP*GP*AP*GP*GP*GP*AP*AP*T)-3') | polymer | 19 | 6013.9 | 1 | synthetic construct | ||
| 8 | I (A) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 1 | Chemie (SF4) | |||
| 9 | J (A) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 10 | K (A) | 2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE | non-polymer | 467.2 | 1 | Chemie (DCP) | |||
| 11 | L, M, N, O, P... (A, D, E, F, G...) | water | water | 18.0 | 437 | Chemie (HOH) |
Sequence modifications
A: 1 - 1504 (UniProt: P14284)
F: 1 - 487 (UniProt: A0A6A5PTG9)
| PDB | External Database | Details |
|---|---|---|
| Met -33 | - | initiating methionine |
| Asp -32 | - | expression tag |
| Tyr -31 | - | expression tag |
| Lys -30 | - | expression tag |
| Asp -29 | - | expression tag |
| Asp -28 | - | expression tag |
| Asp -27 | - | expression tag |
| Asp -26 | - | expression tag |
| Lys -25 | - | expression tag |
| Gly -24 | - | expression tag |
| Asp -23 | - | expression tag |
| His -22 | - | expression tag |
| Asn -21 | - | expression tag |
| His -20 | - | expression tag |
| Arg -19 | - | expression tag |
| His -18 | - | expression tag |
| Lys -17 | - | expression tag |
| His -16 | - | expression tag |
| Gly -15 | - | expression tag |
| Asp -14 | - | expression tag |
| Pro -13 | - | expression tag |
| Leu -12 | - | expression tag |
| Glu -11 | - | expression tag |
| Val -10 | - | expression tag |
| Leu -9 | - | expression tag |
| Phe -8 | - | expression tag |
| Gln -7 | - | expression tag |
| Gly -6 | - | expression tag |
| Pro -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Pro -1 | - | expression tag |
| His 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -6 | - | expression tag |
| Pro -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Leu -1 | - | expression tag |
| His 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 452605.1 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 858.9 | |
| All* | Total formula weight | 453464.0 |
