8TLT

Cryo-EM structure of Rev1(deltaN)-Polzeta-DNA-dCTP complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA polymerase zeta catalytic subunit	polymer	1538	177068.5	1	UniProt (P14284) Pfam (PF03104) Pfam (PF00136) Pfam (PF14260) In PDB	Saccharomyces cerevisiae (brewer's yeast)	Protein reversionless 3
2	D, E	DNA polymerase zeta processivity subunit	polymer	245	28791.7	2	UniProt (P38927) Pfam (PF02301) In PDB	Saccharomyces cerevisiae (brewer's yeast)	Revertibility protein 7
3	F	DNA polymerase delta small subunit	polymer	494	55987.4	1	UniProt (A0A6A5PTG9) Pfam (PF04042) Pfam (PF18018) UniProt (by SIFTS) (P46957) In PDB	Saccharomyces cerevisiae (brewer's yeast)	SX2_G0007820.mRNA.1.CDS.1
4	G	DNA polymerase delta subunit 3	polymer	350	40377.7	1	UniProt (P47110) In PDB	Saccharomyces cerevisiae (brewer's yeast)
5	B	DNA repair protein REV1	polymer	985	112384.2	1	UniProt (P12689) Pfam (PF16589) Pfam (PF00817) Pfam (PF21999) Pfam (PF11799) In PDB	Saccharomyces cerevisiae (brewer's yeast)	Reversionless protein 1
6	P	DNA (5'-D(P*CP*CP*CP*TP*CP*CP*CP*CP*TP*AP*C)-3')	polymer	11	3190.1	1		synthetic construct
7	T	DNA (5'-D(*TP*AP*AP*TP*GP*GP*TP*AP*GP*GP*GP*GP*AP*GP*GP*GP*AP*AP*T)-3')	polymer	19	6013.9	1		synthetic construct
8	A	IRON/SULFUR CLUSTER	non-polymer		351.6	1	Chemie (SF4)
9	A	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
10	A	2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE	non-polymer		467.2	1	Chemie (DCP)
11		water	water		18.0	437	Chemie (HOH)

Sequence modifications

A: 1 - 1504 (UniProt: P14284)

PDB	External Database	Details
Met -33	-	initiating methionine
Asp -32	-	expression tag
Tyr -31	-	expression tag
Lys -30	-	expression tag
Asp -29	-	expression tag
Asp -28	-	expression tag
Asp -27	-	expression tag
Asp -26	-	expression tag
Lys -25	-	expression tag
Gly -24	-	expression tag
Asp -23	-	expression tag
His -22	-	expression tag
Asn -21	-	expression tag
His -20	-	expression tag
Arg -19	-	expression tag
His -18	-	expression tag
Lys -17	-	expression tag
His -16	-	expression tag
Gly -15	-	expression tag
Asp -14	-	expression tag
Pro -13	-	expression tag
Leu -12	-	expression tag
Glu -11	-	expression tag
Val -10	-	expression tag
Leu -9	-	expression tag
Phe -8	-	expression tag
Gln -7	-	expression tag
Gly -6	-	expression tag
Pro -5	-	expression tag
Gly -4	-	expression tag
Gly -3	-	expression tag
Asp -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag

F: 1 - 487 (UniProt: A0A6A5PTG9)

PDB	External Database	Details
Gly -6	-	expression tag
Pro -5	-	expression tag
Gly -4	-	expression tag
Gly -3	-	expression tag
Asp -2	-	expression tag
Leu -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	452605.1
Non-Polymers*	Number of molecules	3
	Total formula weight	858.9
All*	Total formula weight	453464.0

*Water molecules are not included.