8TH3

Structure of AT118-H Nanobody Antagonist in Complex with the Angiotensin II Type I Receptor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	AT118-H nanobody, Type-1 angiotensin II receptor, Soluble cytochrome b562 complex	polymer	582	65364.7	2	UniProt (P30556) UniProt (P0ABE7) Pfam (PF00001)	Camelidae	AT1AR,AT1BR,Angiotensin II type-1 receptor,AT1 receptor,Cytochrome b-562
2	C (C)	BAG2 Anti-BRIL Fab Heavy Chain	polymer	231	24539.3	1		synthetic construct
3	D (D)	BAG2 Anti-BRIL Fab Light Chain	polymer	215	23541.2	1		synthetic construct
4	E, F (A)	CHOLESTEROL HEMISUCCINATE	non-polymer		486.7	2	Chemie (Y01)

Sequence modifications

A, B: -134 - 1 (PDB: 8TH3)
A, B: 2 - 226 (UniProt: P30556)
A, B: 227 - 330 (UniProt: P0ABE7)

PDB	External Database	Details
Trp 232	Met 29	engineered mutation
Ile 327	His 124	engineered mutation
Leu 331	-	linker
Glu 332	-	linker
Arg 333	-	linker
Ala 334	-	linker
Arg 335	-	linker
Ser 336	-	linker
Thr 337	-	linker
Leu 338	-	linker
Asp 339	-	linker
Lys 340	-	linker
Leu 341	-	linker

A, B: 1235 - 1319 (UniProt: P30556)

PDB	External Database	Details
Gly 1320	-	expression tag
Gly 1321	-	expression tag
Ser 1322	-	expression tag
Ser 1323	-	expression tag
Leu 1324	-	expression tag
Glu 1325	-	expression tag
Val 1326	-	expression tag
Leu 1327	-	expression tag
Phe 1328	-	expression tag
Gln 1329	-	expression tag
Gly 1330	-	expression tag
Pro 1331	-	expression tag
Thr 1332	-	expression tag
Glu 1333	-	expression tag
Thr 1334	-	expression tag
Ser 1335	-	expression tag
Gln 1336	-	expression tag
Val 1337	-	expression tag
Ala 1338	-	expression tag
Pro 1339	-	expression tag
Ala 1340	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	178809.8
Non-Polymers*	Number of molecules	2
	Total formula weight	973.5
All*	Total formula weight	179783.3

*Water molecules are not included.