8TH1

Crystal Structure of the G3BP1 NTF2-like domain bound to the IDR1 of SARS-CoV-2 nucleocapsid protein D3L mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Ras GTPase-activating protein-binding protein 1	polymer	164	18515.9	4	UniProt (Q13283) Pfam (PF02136) In PDB	Homo sapiens (human)	G3BP-1,ATP-dependent DNA helicase VIII,hDH VIII,GAP SH3 domain-binding protein 1
2	E, F, G, H	Nucleoprotein	polymer	25	2604.8	4	UniProt (P0DTC9) In PDB	Severe acute respiratory syndrome coronavirus 2	N,Nucleocapsid protein,NC,Protein N
3		water	water		18.0	284	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 139 (UniProt: Q13283)

PDB	External Database	Details
Met -24	-	expression tag
Ser -23	-	expression tag
Leu -22	-	expression tag
Asn -21	-	expression tag
Gly -20	-	expression tag
Pro -19	-	expression tag
Gln -18	-	expression tag
Asn -17	-	expression tag
Gln -16	-	expression tag
Arg -15	-	expression tag
Asn -14	-	expression tag
Ala -13	-	expression tag
Pro -12	-	expression tag
Arg -11	-	expression tag
Ile -10	-	expression tag
Thr -9	-	expression tag
Phe -8	-	expression tag
Gly -7	-	expression tag
Gly -6	-	expression tag
Pro -5	-	expression tag
Ser -4	-	expression tag
Asp -3	-	expression tag
Ser -2	-	expression tag
Thr -1	-	expression tag
Ser 0	-	expression tag

E, F, G, H: 1 - 25 (UniProt: P0DTC9)

PDB	External Database	Details
Leu 3	Asp 3	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	84482.9
All*	Total formula weight	84482.9

*Water molecules are not included.