8TH1
Crystal Structure of the G3BP1 NTF2-like domain bound to the IDR1 of SARS-CoV-2 nucleocapsid protein D3L mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-13 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 1.03319 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.880, 85.890, 70.690 |
Unit cell angles | 90.00, 110.50, 90.00 |
Refinement procedure
Resolution | 48.720 - 1.800 |
R-factor | 0.20009 |
Rwork | 0.198 |
R-free | 0.23828 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3q90 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.608 |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.720 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 1.106 | |
Number of reflections | 51633 | 3223 |
<I/σ(I)> | 12.9 | |
Completeness [%] | 99.0 | |
Redundancy | 6.8 | |
CC(1/2) | 0.956 | 0.763 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.2 M sodium thiocyanate, 20% PEG 3350 |