8T60

CryoEM structure of an inward-facing MelBSt at a Na(+)-bound and sugar low-affinity conformation

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Melibiose permease	polymer	485	54104.4	1	UniProt (P30878) Pfam (PF13347) In PDB	Salmonella enterica subsp. enterica serovar Typhimurium	Melibiose carrier,Melibiose transporter,Melibiose/cation symporter,Na+ (Li+)/melibiose symporter,Thiomethylgalactoside permease II
2	B	Nb725_4	polymer	134	14817.5	1		synthetic construct
3	H	NabFab_H Chain	polymer	239	25684.5	1		synthetic construct
4	L	NabFab_L Chain	polymer	215	23258.8	1		synthetic construct
5	A	SODIUM ION	non-polymer		23.0	1	Chemie (NA)

Sequence modifications

A: 2 - 476 (UniProt: P30878)

PDB	External Database	Details
Met 5	Leu 5	conflict
His 477	-	expression tag
His 478	-	expression tag
His 479	-	expression tag
His 480	-	expression tag
His 481	-	expression tag
His 482	-	expression tag
His 483	-	expression tag
His 484	-	expression tag
His 485	-	expression tag
His 486	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	117865.2
Non-Polymers*	Number of molecules	1
	Total formula weight	23.0
All*	Total formula weight	117888.2

*Water molecules are not included.