8T60
CryoEM structure of an inward-facing MelBSt at a Na(+)-bound and sugar low-affinity conformation
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK II |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Bioquantum |
| Detector | FEI CETA (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.29 |
| Software | cryoSPARC,PHENIX,EPU,UCSF ChimeraX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
