8T60
CryoEM structure of an inward-facing MelBSt at a Na(+)-bound and sugar low-affinity conformation
This is a non-PDB format compatible entry.
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK II |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Energy Filter | GIF Bioquantum |
Detector | FEI CETA (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.29 |
Software | cryoSPARC,PHENIX,EPU,UCSF ChimeraX |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |