8T2D

Ubiquitin variant i53:Mutant T12Y.T14E.L67R with 53BP1 Tudor domain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Ubiquitin variant i53	polymer	78	8794.1	1	UniProt (J3QS39) Pfam (PF00240) UniProt (by SIFTS) (P0CG47)	Homo sapiens (human)
2	B (A)	Tumor protein p53 binding protein 1	polymer	124	13916.7	1	UniProt (A6NNK5) Pfam (PF09038) UniProt (by SIFTS) (Q12888)	Homo sapiens (human)
3	C, D (B, A)	water	water		18.0	38	Chemie (HOH)

Sequence modifications

B: 1 - 74 (UniProt: J3QS39)

PDB	External Database	Details
Gly -3	-	expression tag
Pro -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Leu 2	Gln 2	conflict
Tyr 12	Thr 12	engineered mutation
Glu 14	Thr 14	engineered mutation
Ala 44	Ile 44	conflict
Ser 49	Gln 49	conflict
Leu 62	Gln 62	conflict
Asp 64	Glu 64	conflict
Lys 66	Thr 66	conflict
Arg 67	Leu 67	engineered mutation
Pro 69	Leu 69	conflict
Leu 70	Val 70	conflict

A: 1484 - 1603 (UniProt: A6NNK5)

PDB	External Database	Details
Gly 1480	-	expression tag
Pro 1481	-	expression tag
Gly 1482	-	expression tag
Ser 1483	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	22710.8
All*	Total formula weight	22710.8

*Water molecules are not included.