8T1C
Cryo-EM structure of human TRPV4 ankyrin repeat domain in complex with GTPase RhoA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Transient receptor potential cation channel subfamily V member 4-Enhanced green fluorescent protein chimera | polymer | 1132 | 127717.9 | 1 | UniProt (Q9HBA0) UniProt (C5MKY7) Pfam (PF00023) Pfam (PF00520) | Homo sapiens (human) | TrpV4,Osm-9-like TRP channel 4,OTRPC4,Transient receptor potential protein 12,TRP12,Vanilloid receptor-like channel 2,Vanilloid receptor-like protein 2,VRL-2,Vanilloid receptor-related osmotically-activated channel,VR-OAC |
| 2 | B (E) | Transforming protein RhoA | polymer | 193 | 21799.2 | 1 | UniProt (P61586) Pfam (PF00071) | Homo sapiens (human) | Rho cDNA clone 12,h12 |
| 3 | C (E) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537) |
Sequence modifications
A: 1 - 871 (UniProt: Q9HBA0)
A: 882 - 1119 (UniProt: C5MKY7)
| PDB | External Database | Details |
|---|---|---|
| Leu 872 | - | linker |
| Val 873 | - | linker |
| Pro 874 | - | linker |
| Arg 875 | - | linker |
| Gly 876 | - | linker |
| Ser 877 | - | linker |
| Ala 878 | - | linker |
| Ala 879 | - | linker |
| Ala 880 | - | linker |
| Ala 881 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Lys 1087 | Ala 207 | engineered mutation |
| Ser 1120 | - | expression tag |
| Gly 1121 | - | expression tag |
| Leu 1122 | - | expression tag |
| Arg 1123 | - | expression tag |
| Ser 1124 | - | expression tag |
| Trp 1125 | - | expression tag |
| Ser 1126 | - | expression tag |
| His 1127 | - | expression tag |
| Pro 1128 | - | expression tag |
| Gln 1129 | - | expression tag |
| Phe 1130 | - | expression tag |
| Glu 1131 | - | expression tag |
| Lys 1132 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 149517.1 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 443.2 | |
| All* | Total formula weight | 149960.3 |
