8T1C

Cryo-EM structure of human TRPV4 ankyrin repeat domain in complex with GTPase RhoA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Transient receptor potential cation channel subfamily V member 4-Enhanced green fluorescent protein chimera	polymer	1132	127717.9	1	UniProt (Q9HBA0) UniProt (C5MKY7) Pfam (PF00023) Pfam (PF00520)	Homo sapiens (human)	TrpV4,Osm-9-like TRP channel 4,OTRPC4,Transient receptor potential protein 12,TRP12,Vanilloid receptor-like channel 2,Vanilloid receptor-like protein 2,VRL-2,Vanilloid receptor-related osmotically-activated channel,VR-OAC
2	B (E)	Transforming protein RhoA	polymer	193	21799.2	1	UniProt (P61586) Pfam (PF00071)	Homo sapiens (human)	Rho cDNA clone 12,h12
3	C (E)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP)

Sequence modifications

A: 1 - 871 (UniProt: Q9HBA0)

PDB	External Database	Details
Leu 872	-	linker
Val 873	-	linker
Pro 874	-	linker
Arg 875	-	linker
Gly 876	-	linker
Ser 877	-	linker
Ala 878	-	linker
Ala 879	-	linker
Ala 880	-	linker
Ala 881	-	linker

A: 882 - 1119 (UniProt: C5MKY7)

PDB	External Database	Details
Lys 1087	Ala 207	engineered mutation
Ser 1120	-	expression tag
Gly 1121	-	expression tag
Leu 1122	-	expression tag
Arg 1123	-	expression tag
Ser 1124	-	expression tag
Trp 1125	-	expression tag
Ser 1126	-	expression tag
His 1127	-	expression tag
Pro 1128	-	expression tag
Gln 1129	-	expression tag
Phe 1130	-	expression tag
Glu 1131	-	expression tag
Lys 1132	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	149517.1
Non-Polymers*	Number of molecules	1
	Total formula weight	443.2
All*	Total formula weight	149960.3

*Water molecules are not included.