8T1C
Cryo-EM structure of human TRPV4 ankyrin repeat domain in complex with GTPase RhoA
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Transient receptor potential cation channel subfamily V member 4-Enhanced green fluorescent protein chimera | polymer | 1132 | 127717.9 | 1 | UniProt (Q9HBA0) UniProt (C5MKY7) Pfam (PF00023) Pfam (PF00520) In PDB | Homo sapiens (human) | TrpV4,Osm-9-like TRP channel 4,OTRPC4,Transient receptor potential protein 12,TRP12,Vanilloid receptor-like channel 2,Vanilloid receptor-like protein 2,VRL-2,Vanilloid receptor-related osmotically-activated channel,VR-OAC |
2 | E | Transforming protein RhoA | polymer | 193 | 21799.2 | 1 | UniProt (P61586) Pfam (PF00071) In PDB | Homo sapiens (human) | Rho cDNA clone 12,h12 |
3 | E | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) |
Sequence modifications
A: 1 - 871 (UniProt: Q9HBA0)
A: 882 - 1119 (UniProt: C5MKY7)
PDB | External Database | Details |
---|---|---|
Leu 872 | - | linker |
Val 873 | - | linker |
Pro 874 | - | linker |
Arg 875 | - | linker |
Gly 876 | - | linker |
Ser 877 | - | linker |
Ala 878 | - | linker |
Ala 879 | - | linker |
Ala 880 | - | linker |
Ala 881 | - | linker |
PDB | External Database | Details |
---|---|---|
Lys 1087 | Ala 207 | engineered mutation |
Ser 1120 | - | expression tag |
Gly 1121 | - | expression tag |
Leu 1122 | - | expression tag |
Arg 1123 | - | expression tag |
Ser 1124 | - | expression tag |
Trp 1125 | - | expression tag |
Ser 1126 | - | expression tag |
His 1127 | - | expression tag |
Pro 1128 | - | expression tag |
Gln 1129 | - | expression tag |
Phe 1130 | - | expression tag |
Glu 1131 | - | expression tag |
Lys 1132 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 149517.1 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 443.2 | |
All* | Total formula weight | 149960.3 |