Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

8T1C

Cryo-EM structure of human TRPV4 ankyrin repeat domain in complex with GTPase RhoA

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000122	biological_process	negative regulation of transcription by RNA polymerase II
A	0000166	molecular_function	nucleotide binding
A	0001666	biological_process	response to hypoxia
A	0002024	biological_process	diet induced thermogenesis
A	0003779	molecular_function	actin binding
A	0005034	molecular_function	osmosensor activity
A	0005080	molecular_function	protein kinase C binding
A	0005216	molecular_function	monoatomic ion channel activity
A	0005261	molecular_function	monoatomic cation channel activity
A	0005262	molecular_function	calcium channel activity
A	0005515	molecular_function	protein binding
A	0005516	molecular_function	calmodulin binding
A	0005524	molecular_function	ATP binding
A	0005783	cellular_component	endoplasmic reticulum
A	0005881	cellular_component	cytoplasmic microtubule
A	0005886	cellular_component	plasma membrane
A	0005912	cellular_component	adherens junction
A	0005925	cellular_component	focal adhesion
A	0005929	cellular_component	cilium
A	0006091	biological_process	generation of precursor metabolites and energy
A	0006811	biological_process	monoatomic ion transport
A	0006816	biological_process	calcium ion transport
A	0006874	biological_process	intracellular calcium ion homeostasis
A	0006884	biological_process	cell volume homeostasis
A	0006970	biological_process	response to osmotic stress
A	0006971	biological_process	hypotonic response
A	0007015	biological_process	actin filament organization
A	0007043	biological_process	cell-cell junction assembly
A	0007204	biological_process	positive regulation of cytosolic calcium ion concentration
A	0007231	biological_process	osmosensory signaling pathway
A	0008017	molecular_function	microtubule binding
A	0008218	biological_process	bioluminescence
A	0008289	molecular_function	lipid binding
A	0009612	biological_process	response to mechanical stimulus
A	0009986	cellular_component	cell surface
A	0010628	biological_process	positive regulation of gene expression
A	0010759	biological_process	positive regulation of macrophage chemotaxis
A	0010977	biological_process	negative regulation of neuron projection development
A	0015275	molecular_function	stretch-activated, monoatomic cation-selective, calcium channel activity
A	0016020	cellular_component	membrane
A	0016324	cellular_component	apical plasma membrane
A	0019901	molecular_function	protein kinase binding
A	0030027	cellular_component	lamellipodium
A	0030036	biological_process	actin cytoskeleton organization
A	0030103	biological_process	vasopressin secretion
A	0030175	cellular_component	filopodium
A	0030426	cellular_component	growth cone
A	0030864	cellular_component	cortical actin cytoskeleton
A	0031117	biological_process	positive regulation of microtubule depolymerization
A	0032587	cellular_component	ruffle membrane
A	0032755	biological_process	positive regulation of interleukin-6 production
A	0032868	biological_process	response to insulin
A	0034220	biological_process	monoatomic ion transmembrane transport
A	0034605	biological_process	cellular response to heat
A	0042169	molecular_function	SH2 domain binding
A	0042538	biological_process	hyperosmotic salinity response
A	0042593	biological_process	glucose homeostasis
A	0042802	molecular_function	identical protein binding
A	0042995	cellular_component	cell projection
A	0043014	molecular_function	alpha-tubulin binding
A	0043117	biological_process	positive regulation of vascular permeability
A	0043622	biological_process	cortical microtubule organization
A	0045989	biological_process	positive regulation of striated muscle contraction
A	0046330	biological_process	positive regulation of JNK cascade
A	0046785	biological_process	microtubule polymerization
A	0046872	molecular_function	metal ion binding
A	0047484	biological_process	regulation of response to osmotic stress
A	0048487	molecular_function	beta-tubulin binding
A	0050729	biological_process	positive regulation of inflammatory response
A	0050891	biological_process	multicellular organismal-level water homeostasis
A	0051015	molecular_function	actin filament binding
A	0055085	biological_process	transmembrane transport
A	0060351	biological_process	cartilage development involved in endochondral bone morphogenesis
A	0070161	cellular_component	anchoring junction
A	0070374	biological_process	positive regulation of ERK1 and ERK2 cascade
A	0070509	biological_process	calcium ion import
A	0070588	biological_process	calcium ion transmembrane transport
A	0071470	biological_process	cellular response to osmotic stress
A	0071476	biological_process	cellular hypotonic response
A	0071477	biological_process	cellular hypotonic salinity response
A	0071639	biological_process	positive regulation of monocyte chemotactic protein-1 production
A	0071642	biological_process	positive regulation of macrophage inflammatory protein 1 alpha production
A	0071651	biological_process	positive regulation of chemokine (C-C motif) ligand 5 production
A	0097009	biological_process	energy homeostasis
A	0097497	biological_process	blood vessel endothelial cell delamination
A	0098703	biological_process	calcium ion import across plasma membrane
A	1902656	biological_process	calcium ion import into cytosol
A	1903444	biological_process	negative regulation of brown fat cell differentiation
A	1903715	biological_process	regulation of aerobic respiration
A	2000340	biological_process	positive regulation of chemokine (C-X-C motif) ligand 1 production
E	0000166	molecular_function	nucleotide binding
E	0000281	biological_process	mitotic cytokinesis
E	0000902	biological_process	cell morphogenesis
E	0001764	biological_process	neuron migration
E	0001822	biological_process	kidney development
E	0001998	biological_process	angiotensin-mediated vasoconstriction involved in regulation of systemic arterial blood pressure
E	0002363	biological_process	alpha-beta T cell lineage commitment
E	0003100	biological_process	regulation of systemic arterial blood pressure by endothelin
E	0003189	biological_process	aortic valve formation
E	0003924	molecular_function	GTPase activity
E	0003925	molecular_function	G protein activity
E	0005515	molecular_function	protein binding
E	0005525	molecular_function	GTP binding
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0005768	cellular_component	endosome
E	0005789	cellular_component	endoplasmic reticulum membrane
E	0005829	cellular_component	cytosol
E	0005856	cellular_component	cytoskeleton
E	0005886	cellular_component	plasma membrane
E	0005925	cellular_component	focal adhesion
E	0005938	cellular_component	cell cortex
E	0006357	biological_process	regulation of transcription by RNA polymerase II
E	0007010	biological_process	cytoskeleton organization
E	0007155	biological_process	cell adhesion
E	0007160	biological_process	cell-matrix adhesion
E	0007264	biological_process	small GTPase-mediated signal transduction
E	0007266	biological_process	Rho protein signal transduction
E	0007519	biological_process	skeletal muscle tissue development
E	0009898	cellular_component	cytoplasmic side of plasma membrane
E	0010812	biological_process	negative regulation of cell-substrate adhesion
E	0010975	biological_process	regulation of neuron projection development
E	0016020	cellular_component	membrane
E	0016477	biological_process	cell migration
E	0016787	molecular_function	hydrolase activity
E	0017022	molecular_function	myosin binding
E	0019901	molecular_function	protein kinase binding
E	0021762	biological_process	substantia nigra development
E	0021795	biological_process	cerebral cortex cell migration
E	0021861	biological_process	forebrain radial glial cell differentiation
E	0030027	cellular_component	lamellipodium
E	0030036	biological_process	actin cytoskeleton organization
E	0030054	cellular_component	cell junction
E	0030154	biological_process	cell differentiation
E	0030334	biological_process	regulation of cell migration
E	0030335	biological_process	positive regulation of cell migration
E	0030425	cellular_component	dendrite
E	0030496	cellular_component	midbody
E	0030521	biological_process	androgen receptor signaling pathway
E	0030667	cellular_component	secretory granule membrane
E	0031122	biological_process	cytoplasmic microtubule organization
E	0031982	cellular_component	vesicle
E	0032154	cellular_component	cleavage furrow
E	0032467	biological_process	positive regulation of cytokinesis
E	0032587	cellular_component	ruffle membrane
E	0032956	biological_process	regulation of actin cytoskeleton organization
E	0033144	biological_process	negative regulation of intracellular steroid hormone receptor signaling pathway
E	0033688	biological_process	regulation of osteoblast proliferation
E	0034329	biological_process	cell junction assembly
E	0034446	biological_process	substrate adhesion-dependent cell spreading
E	0035385	biological_process	Roundabout signaling pathway
E	0036089	biological_process	cleavage furrow formation
E	0038027	biological_process	apolipoprotein A-I-mediated signaling pathway
E	0042476	biological_process	odontogenesis
E	0042995	cellular_component	cell projection
E	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
E	0043149	biological_process	stress fiber assembly
E	0043197	cellular_component	dendritic spine
E	0043296	cellular_component	apical junction complex
E	0043297	biological_process	apical junction assembly
E	0043366	biological_process	beta selection
E	0043542	biological_process	endothelial cell migration
E	0043931	biological_process	ossification involved in bone maturation
E	0044319	biological_process	wound healing, spreading of cells
E	0045198	biological_process	establishment of epithelial cell apical/basal polarity
E	0045666	biological_process	positive regulation of neuron differentiation
E	0045792	biological_process	negative regulation of cell size
E	0046638	biological_process	positive regulation of alpha-beta T cell differentiation
E	0050919	biological_process	negative chemotaxis
E	0051301	biological_process	cell division
E	0051496	biological_process	positive regulation of stress fiber assembly
E	0051893	biological_process	regulation of focal adhesion assembly
E	0060071	biological_process	Wnt signaling pathway, planar cell polarity pathway
E	0060193	biological_process	positive regulation of lipase activity
E	0061430	biological_process	bone trabecula morphogenesis
E	0070062	cellular_component	extracellular exosome
E	0070507	biological_process	regulation of microtubule cytoskeleton organization
E	0071222	biological_process	cellular response to lipopolysaccharide
E	0071345	biological_process	cellular response to cytokine stimulus
E	0071526	biological_process	semaphorin-plexin signaling pathway
E	0071803	biological_process	positive regulation of podosome assembly
E	0071902	biological_process	positive regulation of protein serine/threonine kinase activity
E	0071944	cellular_component	cell periphery
E	0090051	biological_process	negative regulation of cell migration involved in sprouting angiogenesis
E	0090307	biological_process	mitotic spindle assembly
E	0090324	biological_process	negative regulation of oxidative phosphorylation
E	0097049	biological_process	motor neuron apoptotic process
E	0097498	biological_process	endothelial tube lumen extension
E	0098794	cellular_component	postsynapse
E	0098978	cellular_component	glutamatergic synapse
E	0099159	biological_process	regulation of modification of postsynaptic structure
E	0101003	cellular_component	ficolin-1-rich granule membrane
E	1901224	biological_process	positive regulation of non-canonical NF-kappaB signal transduction
E	1902766	biological_process	skeletal muscle satellite cell migration
E	1903427	biological_process	negative regulation of reactive oxygen species biosynthetic process
E	1903673	biological_process	mitotic cleavage furrow formation
E	1904695	biological_process	positive regulation of vascular associated smooth muscle contraction
E	1904996	biological_process	positive regulation of leukocyte adhesion to vascular endothelial cell
E	1905274	biological_process	regulation of modification of postsynaptic actin cytoskeleton
E	1990869	biological_process	cellular response to chemokine
E	2000177	biological_process	regulation of neural precursor cell proliferation
E	2000406	biological_process	positive regulation of T cell migration
E	2000672	biological_process	negative regulation of motor neuron apoptotic process

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	29
Details	Repeat: {"description":"ANK 1"}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	29
Details	Repeat: {"description":"ANK 2"}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	7
Details	Binding site: {"evidences":[{"source":"PubMed","id":"22702953","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4DX2","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"A0A1D5PXA5","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	1
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"UniProtKB","id":"Q9EPK8","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	17
Details	Region: {"description":"Switch II region; involved in RAP1GDS1 isoform 2 binding","evidences":[{"source":"PubMed","id":"30190425","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5ZHX","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	10
Details	Binding site: {"evidences":[{"source":"PubMed","id":"10748207","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"12777804","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P62820","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	1
Details	Modified residue: {"description":"(Microbial infection) O-AMP-threonine; by Vibrio VopS","evidences":[{"source":"PubMed","id":"19039103","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	1
Details	Modified residue: {"description":"(Microbial infection) ADP-ribosylasparagine; by botulinum toxin","evidences":[{"source":"PubMed","id":"1328215","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI11
Number of Residues	1
Details	Modified residue: {"description":"5-glutamyl serotonin","evidences":[{"source":"UniProtKB","id":"Q9QUI0","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI12
Number of Residues	1
Details	Glycosylation: {"description":"(Microbial infection) O-linked (Glc) threonine; by C.difficile toxins TcdA and TcdB; alternate","evidences":[{"source":"PubMed","id":"24905543","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"7775453","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"7777059","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI13
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"23871831","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)