8SVE

Structure of Monomeric Interleukin-10 Grafted into and Antibody CDR

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (H, I)	Fab Heavy chain	polymer	227	24349.4	2	Pfam (PF07686) Pfam (PF07654) UniProt (by SIFTS) (P0DOX5)	Homo sapiens (human)
2	C, D (L, M)	Interleukin-10,Fab light chain	polymer	379	42172.5	2	UniProt (P22301) Pfam (PF00726)	Homo sapiens (human)	IL-10,Cytokine synthesis inhibitory factor,CSIF
3	E (H)	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
4	F, G, H, I, J (I, L, M)	1,2-ETHANEDIOL	non-polymer		62.1	5	Chemie (EDO)
5	K, L, M, N (H, I, L, M)	water	water		18.0	233	Chemie (HOH)

Sequence modifications

L, M: 29 - 194 (UniProt: P22301)

PDB	External Database	Details
Asp 1	-	expression tag
Ile 2	-	expression tag
Gln 3	-	expression tag
Met 4	-	expression tag
Thr 5	-	expression tag
Gln 6	-	expression tag
Ser 7	-	expression tag
Pro 8	-	expression tag
Ser 9	-	expression tag
Thr 10	-	expression tag
Leu 11	-	expression tag
Ser 12	-	expression tag
Ala 13	-	expression tag
Ser 14	-	expression tag
Val 15	-	expression tag
Gly 16	-	expression tag
Asp 17	-	expression tag
Arg 18	-	expression tag
Val 19	-	expression tag
Thr 20	-	expression tag
Ile 21	-	expression tag
Thr 22	-	expression tag
Cys 23	-	expression tag
Lys 24	-	expression tag
Ala 25	-	expression tag
Gln 26	-	expression tag
Leu 27	-	expression tag
Ser 28	-	expression tag
Gly 145	-	insertion
Gly 146	-	insertion
Gly 147	-	insertion
Ser 148	-	insertion
Gly 149	-	insertion
Gly 150	-	insertion

L, M: 195 - 379 (PDB: 8SVE)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	133043.9
Non-Polymers*	Number of molecules	6
	Total formula weight	369.4
All*	Total formula weight	133413.3

*Water molecules are not included.