8SVE
Structure of Monomeric Interleukin-10 Grafted into and Antibody CDR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-07-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97648 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 80.641, 104.662, 82.826 |
Unit cell angles | 90.00, 115.29, 90.00 |
Refinement procedure
Resolution | 42.900 - 2.400 |
R-factor | 0.1906 |
Rwork | 0.188 |
R-free | 0.23230 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.855 |
Data reduction software | DENZO (2.3.10) |
Data scaling software | SCALEPACK (2.3.10) |
Phasing software | PHASER (2.6.1) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.122 | 0.794 |
Rmeas | 0.145 | 0.953 |
Rpim | 0.077 | 0.521 |
Number of reflections | 47411 | 3890 |
<I/σ(I)> | 9.4 | 1.12 |
Completeness [%] | 96.9 | 79.9 |
Redundancy | 3.3 | 2.9 |
CC(1/2) | 0.646 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.9 | 294 | 20% PEG 3350, 0.2M Magnesium Acetate, pH 7.9 |