8SSZ

The 2alpha3beta stoichiometry of full-length human alpha4beta2 nicotinic acetylcholine receptor in complex with acetylcholine and calcium

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D	Neuronal acetylcholine receptor subunit alpha-4	polymer	601	67411.8	2	UniProt (P43681) Pfam (PF02931) Pfam (PF02932) In PDB	Homo sapiens (human)
2	B, C, E	Neuronal acetylcholine receptor subunit beta-2	polymer	487	55650.0	3	UniProt (P17787) Pfam (PF02931) Pfam (PF02932) In PDB	Homo sapiens (human)
3	F, H, J	IgG1 Kappa Light Chain	polymer	238	26378.6	3		Mus musculus (mouse)
4	G, I, K	IgG1 Heavy Chain	polymer	462	51195.7	3		Mus musculus (mouse)
5	L, M, N	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	3	In PDB GlyTouCan (G81315DD)
6	A, D	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)
7	A, D	ACETYLCHOLINE	non-polymer		146.2	2	Chemie (ACH)
8	A, D	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
9	E	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
10	E	N-OCTANE	non-polymer		114.2	1	Chemie (OCT)
11		water	water		18.0	7	Chemie (HOH)

Sequence modifications

B, C, E: 1 - 477 (UniProt: P17787)

PDB	External Database	Details
Ser 478	-	linker
Ala 479	-	linker
Trp 480	-	expression tag
Ser 481	-	expression tag
His 482	-	expression tag
Pro 483	-	expression tag
Gln 484	-	expression tag
Phe 485	-	expression tag
Glu 486	-	expression tag
Lys 487	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	534496.2
Branched	Number of molecules	3
	Total formula weight	2246.0
Non-Polymers*	Number of molecules	12
	Total formula weight	1837.0
All*	Total formula weight	538579.3

*Water molecules are not included.