Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

8SSZ

The 2alpha3beta stoichiometry of full-length human alpha4beta2 nicotinic acetylcholine receptor in complex with acetylcholine and calcium

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004888	molecular_function	transmembrane signaling receptor activity
A	0005216	molecular_function	monoatomic ion channel activity
A	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
A	0006811	biological_process	monoatomic ion transport
A	0016020	cellular_component	membrane
A	0022848	molecular_function	acetylcholine-gated monoatomic cation-selective channel activity
A	0034220	biological_process	monoatomic ion transmembrane transport
A	0045211	cellular_component	postsynaptic membrane
B	0001666	biological_process	response to hypoxia
B	0004888	molecular_function	transmembrane signaling receptor activity
B	0005216	molecular_function	monoatomic ion channel activity
B	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
B	0005515	molecular_function	protein binding
B	0005886	cellular_component	plasma membrane
B	0005892	cellular_component	acetylcholine-gated channel complex
B	0006811	biological_process	monoatomic ion transport
B	0006816	biological_process	calcium ion transport
B	0006939	biological_process	smooth muscle contraction
B	0007154	biological_process	cell communication
B	0007165	biological_process	signal transduction
B	0007271	biological_process	synaptic transmission, cholinergic
B	0007601	biological_process	visual perception
B	0007605	biological_process	sensory perception of sound
B	0007612	biological_process	learning
B	0007613	biological_process	memory
B	0007626	biological_process	locomotory behavior
B	0008306	biological_process	associative learning
B	0008542	biological_process	visual learning
B	0009897	cellular_component	external side of plasma membrane
B	0014059	biological_process	regulation of dopamine secretion
B	0015276	molecular_function	ligand-gated monoatomic ion channel activity
B	0015464	molecular_function	acetylcholine receptor activity
B	0016020	cellular_component	membrane
B	0019233	biological_process	sensory perception of pain
B	0021562	biological_process	vestibulocochlear nerve development
B	0021631	biological_process	optic nerve morphogenesis
B	0021771	biological_process	lateral geniculate nucleus development
B	0021952	biological_process	central nervous system projection neuron axonogenesis
B	0022848	molecular_function	acetylcholine-gated monoatomic cation-selective channel activity
B	0023052	biological_process	signaling
B	0030890	biological_process	positive regulation of B cell proliferation
B	0032225	biological_process	regulation of synaptic transmission, dopaminergic
B	0032991	cellular_component	protein-containing complex
B	0033603	biological_process	positive regulation of dopamine secretion
B	0034220	biological_process	monoatomic ion transmembrane transport
B	0035094	biological_process	response to nicotine
B	0035095	biological_process	behavioral response to nicotine
B	0035176	biological_process	social behavior
B	0042053	biological_process	regulation of dopamine metabolic process
B	0042113	biological_process	B cell activation
B	0042166	molecular_function	acetylcholine binding
B	0042220	biological_process	response to cocaine
B	0042320	biological_process	regulation of circadian sleep/wake cycle, REM sleep
B	0042734	cellular_component	presynaptic membrane
B	0043005	cellular_component	neuron projection
B	0044853	cellular_component	plasma membrane raft
B	0044877	molecular_function	protein-containing complex binding
B	0045202	cellular_component	synapse
B	0045211	cellular_component	postsynaptic membrane
B	0045471	biological_process	response to ethanol
B	0045759	biological_process	negative regulation of action potential
B	0048814	biological_process	regulation of dendrite morphogenesis
B	0050877	biological_process	nervous system process
B	0050890	biological_process	cognition
B	0050997	molecular_function	quaternary ammonium group binding
B	0051899	biological_process	membrane depolarization
B	0051963	biological_process	regulation of synapse assembly
B	0060078	biological_process	regulation of postsynaptic membrane potential
B	0060079	biological_process	excitatory postsynaptic potential
B	0060084	biological_process	synaptic transmission involved in micturition
B	0095500	biological_process	acetylcholine receptor signaling pathway
B	0098981	cellular_component	cholinergic synapse
B	0099634	cellular_component	postsynaptic specialization membrane
B	1901363	molecular_function	heterocyclic compound binding
B	1904315	molecular_function	transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential
B	1905144	biological_process	response to acetylcholine
C	0001666	biological_process	response to hypoxia
C	0004888	molecular_function	transmembrane signaling receptor activity
C	0005216	molecular_function	monoatomic ion channel activity
C	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
C	0005515	molecular_function	protein binding
C	0005886	cellular_component	plasma membrane
C	0005892	cellular_component	acetylcholine-gated channel complex
C	0006811	biological_process	monoatomic ion transport
C	0006816	biological_process	calcium ion transport
C	0006939	biological_process	smooth muscle contraction
C	0007154	biological_process	cell communication
C	0007165	biological_process	signal transduction
C	0007271	biological_process	synaptic transmission, cholinergic
C	0007601	biological_process	visual perception
C	0007605	biological_process	sensory perception of sound
C	0007612	biological_process	learning
C	0007613	biological_process	memory
C	0007626	biological_process	locomotory behavior
C	0008306	biological_process	associative learning
C	0008542	biological_process	visual learning
C	0009897	cellular_component	external side of plasma membrane
C	0014059	biological_process	regulation of dopamine secretion
C	0015276	molecular_function	ligand-gated monoatomic ion channel activity
C	0015464	molecular_function	acetylcholine receptor activity
C	0016020	cellular_component	membrane
C	0019233	biological_process	sensory perception of pain
C	0021562	biological_process	vestibulocochlear nerve development
C	0021631	biological_process	optic nerve morphogenesis
C	0021771	biological_process	lateral geniculate nucleus development
C	0021952	biological_process	central nervous system projection neuron axonogenesis
C	0022848	molecular_function	acetylcholine-gated monoatomic cation-selective channel activity
C	0023052	biological_process	signaling
C	0030890	biological_process	positive regulation of B cell proliferation
C	0032225	biological_process	regulation of synaptic transmission, dopaminergic
C	0032991	cellular_component	protein-containing complex
C	0033603	biological_process	positive regulation of dopamine secretion
C	0034220	biological_process	monoatomic ion transmembrane transport
C	0035094	biological_process	response to nicotine
C	0035095	biological_process	behavioral response to nicotine
C	0035176	biological_process	social behavior
C	0042053	biological_process	regulation of dopamine metabolic process
C	0042113	biological_process	B cell activation
C	0042166	molecular_function	acetylcholine binding
C	0042220	biological_process	response to cocaine
C	0042320	biological_process	regulation of circadian sleep/wake cycle, REM sleep
C	0042734	cellular_component	presynaptic membrane
C	0043005	cellular_component	neuron projection
C	0044853	cellular_component	plasma membrane raft
C	0044877	molecular_function	protein-containing complex binding
C	0045202	cellular_component	synapse
C	0045211	cellular_component	postsynaptic membrane
C	0045471	biological_process	response to ethanol
C	0045759	biological_process	negative regulation of action potential
C	0048814	biological_process	regulation of dendrite morphogenesis
C	0050877	biological_process	nervous system process
C	0050890	biological_process	cognition
C	0050997	molecular_function	quaternary ammonium group binding
C	0051899	biological_process	membrane depolarization
C	0051963	biological_process	regulation of synapse assembly
C	0060078	biological_process	regulation of postsynaptic membrane potential
C	0060079	biological_process	excitatory postsynaptic potential
C	0060084	biological_process	synaptic transmission involved in micturition
C	0095500	biological_process	acetylcholine receptor signaling pathway
C	0098981	cellular_component	cholinergic synapse
C	0099634	cellular_component	postsynaptic specialization membrane
C	1901363	molecular_function	heterocyclic compound binding
C	1904315	molecular_function	transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential
C	1905144	biological_process	response to acetylcholine
D	0004888	molecular_function	transmembrane signaling receptor activity
D	0005216	molecular_function	monoatomic ion channel activity
D	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
D	0006811	biological_process	monoatomic ion transport
D	0016020	cellular_component	membrane
D	0022848	molecular_function	acetylcholine-gated monoatomic cation-selective channel activity
D	0034220	biological_process	monoatomic ion transmembrane transport
D	0045211	cellular_component	postsynaptic membrane
E	0001666	biological_process	response to hypoxia
E	0004888	molecular_function	transmembrane signaling receptor activity
E	0005216	molecular_function	monoatomic ion channel activity
E	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
E	0005515	molecular_function	protein binding
E	0005886	cellular_component	plasma membrane
E	0005892	cellular_component	acetylcholine-gated channel complex
E	0006811	biological_process	monoatomic ion transport
E	0006816	biological_process	calcium ion transport
E	0006939	biological_process	smooth muscle contraction
E	0007154	biological_process	cell communication
E	0007165	biological_process	signal transduction
E	0007271	biological_process	synaptic transmission, cholinergic
E	0007601	biological_process	visual perception
E	0007605	biological_process	sensory perception of sound
E	0007612	biological_process	learning
E	0007613	biological_process	memory
E	0007626	biological_process	locomotory behavior
E	0008306	biological_process	associative learning
E	0008542	biological_process	visual learning
E	0009897	cellular_component	external side of plasma membrane
E	0014059	biological_process	regulation of dopamine secretion
E	0015276	molecular_function	ligand-gated monoatomic ion channel activity
E	0015464	molecular_function	acetylcholine receptor activity
E	0016020	cellular_component	membrane
E	0019233	biological_process	sensory perception of pain
E	0021562	biological_process	vestibulocochlear nerve development
E	0021631	biological_process	optic nerve morphogenesis
E	0021771	biological_process	lateral geniculate nucleus development
E	0021952	biological_process	central nervous system projection neuron axonogenesis
E	0022848	molecular_function	acetylcholine-gated monoatomic cation-selective channel activity
E	0023052	biological_process	signaling
E	0030890	biological_process	positive regulation of B cell proliferation
E	0032225	biological_process	regulation of synaptic transmission, dopaminergic
E	0032991	cellular_component	protein-containing complex
E	0033603	biological_process	positive regulation of dopamine secretion
E	0034220	biological_process	monoatomic ion transmembrane transport
E	0035094	biological_process	response to nicotine
E	0035095	biological_process	behavioral response to nicotine
E	0035176	biological_process	social behavior
E	0042053	biological_process	regulation of dopamine metabolic process
E	0042113	biological_process	B cell activation
E	0042166	molecular_function	acetylcholine binding
E	0042220	biological_process	response to cocaine
E	0042320	biological_process	regulation of circadian sleep/wake cycle, REM sleep
E	0042734	cellular_component	presynaptic membrane
E	0043005	cellular_component	neuron projection
E	0044853	cellular_component	plasma membrane raft
E	0044877	molecular_function	protein-containing complex binding
E	0045202	cellular_component	synapse
E	0045211	cellular_component	postsynaptic membrane
E	0045471	biological_process	response to ethanol
E	0045759	biological_process	negative regulation of action potential
E	0048814	biological_process	regulation of dendrite morphogenesis
E	0050877	biological_process	nervous system process
E	0050890	biological_process	cognition
E	0050997	molecular_function	quaternary ammonium group binding
E	0051899	biological_process	membrane depolarization
E	0051963	biological_process	regulation of synapse assembly
E	0060078	biological_process	regulation of postsynaptic membrane potential
E	0060079	biological_process	excitatory postsynaptic potential
E	0060084	biological_process	synaptic transmission involved in micturition
E	0095500	biological_process	acetylcholine receptor signaling pathway
E	0098981	cellular_component	cholinergic synapse
E	0099634	cellular_component	postsynaptic specialization membrane
E	1901363	molecular_function	heterocyclic compound binding
E	1904315	molecular_function	transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential
E	1905144	biological_process	response to acetylcholine

Functional Information from PROSITE/UniProt

site_id	PS00236
Number of Residues	15
Details	NEUROTR_ION_CHANNEL Neurotransmitter-gated ion-channels signature. CkIeVkhFPfDqqnC

Chain	Residue	Details
B	CYS130-CYS144
A	CYS135-CYS149

site_id	PS00290
Number of Residues	7
Details	IG_MHC Immunoglobulins and major histocompatibility complex proteins signature. YTCEATH

Chain	Residue	Details
F	TYR197-HIS203

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	663
Details	TOPO_DOM: Extracellular => ECO:0000255

Chain	Residue	Details
B	THR1-LYS208
B	LYS260-LYS274
C	THR1-LYS208
C	LYS260-LYS274
E	THR1-LYS208
E	LYS260-LYS274

site_id	SWS_FT_FI2
Number of Residues	246
Details	TRANSMEM: Helical => ECO:0000255

Chain	Residue	Details
B	PRO209-LEU233
E	MET241-SER259
E	TYR275-VAL296
E	ASP434-PHE453
B	MET241-SER259
B	TYR275-VAL296
B	ASP434-PHE453
C	PRO209-LEU233
C	MET241-SER259
C	TYR275-VAL296
C	ASP434-PHE453
E	PRO209-LEU233

site_id	SWS_FT_FI3
Number of Residues	426
Details	TOPO_DOM: Cytoplasmic => ECO:0000255

Chain	Residue	Details
B	PRO234-LYS240
B	HIS297-ILE433
C	PRO234-LYS240
C	HIS297-ILE433
E	PRO234-LYS240
E	HIS297-ILE433

site_id	SWS_FT_FI4
Number of Residues	3
Details	SITE: Key residue that may interfere with effective access of the conotoxin BuIA to the channel binding site => ECO:0000250\|UniProtKB:P12390

Chain	Residue	Details
B	THR59
C	THR59
E	THR59

site_id	SWS_FT_FI5
Number of Residues	6
Details	SITE: Key residue for a rapid dissociation (K(off)) from the conotoxin BuIA => ECO:0000250\|UniProtKB:P12390

Chain	Residue	Details
B	VAL111
B	PHE119
C	VAL111
C	PHE119
E	VAL111
E	PHE119

site_id	SWS_FT_FI6
Number of Residues	9
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255

Chain	Residue	Details
B	ASN26
B	ASN143
B	ASN460
C	ASN26
C	ASN143
C	ASN460
E	ASN26
E	ASN143
E	ASN460

site_id	SWS_FT_FI7
Number of Residues	6
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255

Chain	Residue	Details
A	ASN31
A	ASN81
A	ASN148
D	ASN31
D	ASN81
D	ASN148

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)