8SFO
WT CRISPR-Cas12a with a 20bp R-loop and nontarget strand in the RuvC active site.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated endonuclease Cas12a | polymer | 1311 | 151705.2 | 1 | UniProt (U2UMQ6) Pfam (PF18501) Pfam (PF21918) Pfam (PF18516) Pfam (PF18510) | Acidaminococcus sp. BV3L6 | AsCpf1,CRISPR-associated endonuclease Cpf1 |
| 2 | B (B) | RNA (39-MER) | polymer | 48 | 15352.0 | 1 | synthetic construct | ||
| 3 | C (C) | DNA (40-MER) | polymer | 56 | 17211.1 | 1 | synthetic construct | ||
| 4 | D (D) | DNA (35-MER) | polymer | 56 | 17299.1 | 1 | synthetic construct | ||
| 5 | E, F (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) |
Sequence modifications
A: 1 - 1307 (UniProt: U2UMQ6)
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Ala -1 | - | expression tag |
| Ser 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 201567.4 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 48.6 | |
| All* | Total formula weight | 201616.0 |
