8SFO
WT CRISPR-Cas12a with a 20bp R-loop and nontarget strand in the RuvC active site.
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | CRISPR-associated endonuclease Cas12a | polymer | 1311 | 151705.2 | 1 | UniProt (U2UMQ6) Pfam (PF18501) Pfam (PF21918) Pfam (PF18516) Pfam (PF18510) In PDB | Acidaminococcus sp. BV3L6 | AsCpf1,CRISPR-associated endonuclease Cpf1 |
2 | B | RNA (39-MER) | polymer | 48 | 15352.0 | 1 | synthetic construct | ||
3 | C | DNA (40-MER) | polymer | 56 | 17211.1 | 1 | synthetic construct | ||
4 | D | DNA (35-MER) | polymer | 56 | 17299.1 | 1 | synthetic construct | ||
5 | A | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) |
Sequence modifications
A: 1 - 1307 (UniProt: U2UMQ6)
PDB | External Database | Details |
---|---|---|
Gly -3 | - | expression tag |
Ala -2 | - | expression tag |
Ala -1 | - | expression tag |
Ser 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 201567.4 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 48.6 | |
All* | Total formula weight | 201616.0 |