8SFO
WT CRISPR-Cas12a with a 20bp R-loop and nontarget strand in the RuvC active site.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS GLACIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.3 |
| Software | cryoSPARC,SerialEM |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
