8SFO
WT CRISPR-Cas12a with a 20bp R-loop and nontarget strand in the RuvC active site.
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 8 |
| Details | DNA_BIND: PAM-binding on target DNA => ECO:0000269|PubMed:27114038, ECO:0000269|PubMed:27444870 |
| Chain | Residue | Details |
| A | PRO599-LYS607 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 22 |
| Details | DNA_BIND: Target DNA => ECO:0000269|PubMed:27114038, ECO:0000269|PubMed:27444870 |
| Chain | Residue | Details |
| A | LYS780-GLY783 | |
| A | ARG951-LYS968 | |
| A | SER1051-ALA1053 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 3 |
| Details | ACT_SITE: For pre-crRNA processing => ECO:0000250|UniProtKB:A0Q7Q2 |
| Chain | Residue | Details |
| A | HIS800 | |
| A | LYS809 | |
| A | LYS860 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 3 |
| Details | ACT_SITE: For DNase activity of RuvC domain => ECO:0000269|PubMed:27114038 |
| Chain | Residue | Details |
| A | ASP908 | |
| A | GLU993 | |
| A | ASP1263 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | ACT_SITE: For DNase activity of nuclease domain => ECO:0000269|PubMed:27114038 |
| Chain | Residue | Details |
| A | ARG1226 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:27114038, ECO:0000269|PubMed:27444870 |
| Chain | Residue | Details |
| A | TYR47 | |
| A | ASN175 | |
| A | LYS307 | |
| A | LYS752 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:27114038 |
| Chain | Residue | Details |
| A | MET806 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 3 |
| Details | SITE: Binds crRNA => ECO:0000269|PubMed:27114038, ECO:0000269|PubMed:27444870 |
| Chain | Residue | Details |
| A | ARG18 | |
| A | ARG192 | |
| A | HIS872 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 2 |
| Details | SITE: Binds PAM on target DNA => ECO:0000269|PubMed:27114038, ECO:0000269|PubMed:27444870 |
| Chain | Residue | Details |
| A | THR167 | |
| A | LYS548 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 1 |
| Details | SITE: Binds crRNA-target DNA heteroduplex => ECO:0000269|PubMed:27114038, ECO:0000269|PubMed:27444870 |
| Chain | Residue | Details |
| A | TRP382 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 1 |
| Details | SITE: Binds sequence-specific recognition of both target and non-target strand bases in PAM => ECO:0000269|PubMed:27114038, ECO:0000269|PubMed:27444870 |
| Chain | Residue | Details |
| A | LYS607 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 1 |
| Details | SITE: Binds target DNA => ECO:0000269|PubMed:27114038 |
| Chain | Residue | Details |
| A | GLN1014 |
