8RVO

Proteasomal late precursor complex from pre1-1, state 1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, U (1, M)	Proteasome subunit beta type-6	polymer	241	26905.1	2	UniProt (P23724) Pfam (PF00227)	Saccharomyces cerevisiae (brewer's yeast)	Multicatalytic endopeptidase complex subunit C5,Proteasome component C5
2	B, V (2, N)	Proteasome subunit beta type-7	polymer	266	29471.3	2	UniProt (P30657) Pfam (PF00227)	Saccharomyces cerevisiae (brewer's yeast)	Macropain subunit PRE4,Multicatalytic endopeptidase complex subunit PRE4,Proteasome component PRE4,Proteinase YSCE subunit PRE4
3	C, F (3, 6)	Proteasome maturation factor UMP1	polymer	148	16777.8	2	UniProt (P38293) Pfam (PF05348)	Saccharomyces cerevisiae (brewer's yeast)
4	D, G (4, 7)	Proteasome chaperone 1	polymer	276	30718.1	2	UniProt (Q05778) Pfam (PF10450)	Saccharomyces cerevisiae (brewer's yeast)	Proteasome biogenesis-associated protein 1
5	E, H (5, 8)	Proteasome assembly chaperone 2	polymer	267	30762.9	2	UniProt (P36040) Pfam (PF09754)	Saccharomyces cerevisiae (brewer's yeast)	Alpha-1-proteinase inhibitor-degradation deficient protein 66,Proteasome biogenesis-associated protein 2
6	I, W (A, O)	Proteasome subunit alpha type-1	polymer	252	28033.8	2	UniProt (P21243) Pfam (PF10584) Pfam (PF00227)	Saccharomyces cerevisiae (brewer's yeast)	Macropain subunit C7-alpha,Multicatalytic endopeptidase complex C7,Proteasome component C7-alpha,Proteasome component Y8,Proteinase YSCE subunit 7,SCL1 suppressor protein
7	J, X (B, P)	Proteasome subunit alpha type-2	polymer	250	27191.8	2	UniProt (P23639) Pfam (PF10584) Pfam (PF00227)	Saccharomyces cerevisiae (brewer's yeast)	Macropain subunit Y7,Multicatalytic endopeptidase complex subunit Y7,Proteasome component Y7,Proteinase YSCE subunit 7
8	K, Y (C, Q)	Proteasome subunit alpha type-3	polymer	258	28748.2	2	UniProt (P23638) Pfam (PF10584) Pfam (PF00227)	Saccharomyces cerevisiae (brewer's yeast)	Macropain subunit Y13,Multicatalytic endopeptidase complex subunit Y13,Proteasome component Y13,Proteinase YSCE subunit 13
9	L, Z (D, R)	Proteasome subunit alpha type-4	polymer	254	28478.1	2	UniProt (P40303) Pfam (PF10584) Pfam (PF00227)	Saccharomyces cerevisiae (brewer's yeast)	Macropain subunit PRE6,Multicatalytic endopeptidase complex subunit PRE6,Proteasome component PRE6,Proteinase YSCE subunit PRE6
10	AA, M (E, S)	Proteasome subunit alpha type-5	polymer	260	28649.1	2	UniProt (P32379) Pfam (PF10584) Pfam (PF00227)	Saccharomyces cerevisiae (brewer's yeast)	Macropain subunit PUP2,Multicatalytic endopeptidase complex subunit PUP2,Proteasome component PUP2,Proteinase YSCE subunit PUP2
11	BA, N (F, T)	Proteasome subunit alpha type-6	polymer	234	25634.0	2	UniProt (P40302) Pfam (PF10584) Pfam (PF00227)	Saccharomyces cerevisiae (brewer's yeast)	Macropain subunit PRE5,Multicatalytic endopeptidase complex subunit PRE5,Proteasome component PRE5,Proteinase YSCE subunit PRE5
12	CA, O (G, U)	Probable proteasome subunit alpha type-7	polymer	288	31590.1	2	UniProt (P21242) Pfam (PF10584) Pfam (PF00227)	Saccharomyces cerevisiae (brewer's yeast)	Macropain subunit C1,Multicatalytic endopeptidase complex subunit C1,Proteasome component C1,Proteinase YSCE subunit 1
13	DA, P (H, V)	Proteasome subunit beta type-1	polymer	215	23573.6	2	UniProt (P38624) Pfam (PF00227)	Saccharomyces cerevisiae (brewer's yeast)	Macropain subunit PRE3,Multicatalytic endopeptidase complex subunit PRE3,Proteasome component PRE3,Proteinase YSCE subunit PRE3
14	EA, Q (I, W)	Proteasome subunit beta type-2	polymer	261	28313.9	2	UniProt (P25043) Pfam (PF00227) Pfam (PF12465)	Saccharomyces cerevisiae (brewer's yeast)	Macropain subunit PUP1,Multicatalytic endopeptidase complex subunit PUP1,Proteasome component PUP1,Proteinase YSCE subunit PUP1
15	FA, R (J, X)	Proteasome subunit beta type-3	polymer	205	22627.8	2	UniProt (P25451) Pfam (PF00227)	Saccharomyces cerevisiae (brewer's yeast)	Macropain subunit PUP3,Multicatalytic endopeptidase complex subunit PUP3,Proteasome component PUP3
16	GA, S (K, Y)	Proteasome subunit beta type-4	polymer	212	24431.6	2	UniProt (P22141) Pfam (PF00227)	Saccharomyces cerevisiae (brewer's yeast)	Macropain subunit C11,Multicatalytic endopeptidase complex subunit C11,Proteasome component C11,Proteinase YSCE subunit 11
17	HA, T (L, Z)	Proteasome subunit beta type-5	polymer	287	31670.5	2	UniProt (P30656) Pfam (PF00227)	Saccharomyces cerevisiae (brewer's yeast)	Macropain subunit PRE2,Multicatalytic endopeptidase complex subunit PRE2,Proteasome component PRE2,Proteinase YSCE subunit PRE2
18	AB, BB, CB, DB, EB... (K, L, M, N, O...)	water	water		18.0	1057	Chemie (HOH)

Sequence modifications

G, U: 1 - 288 (UniProt: P21242)

PDB	External Database	Details
Lys 207	Asn 207	conflict

I, W: -29 - 232 (UniProt: P25043)

PDB	External Database	Details
Thr 191	Leu 220	conflict
Pro 192	Thr 221	conflict
Thr 193	-	insertion

K, Y: 1 - 198 (UniProt: P22141)

PDB	External Database	Details
Phe 142	Ser 142	engineered mutation
Asp 199	-	expression tag
Tyr 200	-	expression tag
Lys 201	-	expression tag
Asp 202	-	expression tag
Asp 203	-	expression tag
Asp 204	-	expression tag
Asp 205	-	expression tag
Lys 206	-	expression tag
His 207	-	expression tag
His 208	-	expression tag
His 209	-	expression tag
His 210	-	expression tag
His 211	-	expression tag
His 212	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	34
	Total formula weight	927155.6
All*	Total formula weight	927155.6

*Water molecules are not included.