8RU2

Structure of the F-actin barbed end bound by formin mDia1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B, C, D	Actin, cytoplasmic 1, N-terminally processed	polymer	374	41632.4	3	UniProt (P60709) Pfam (PF00022) In PDB	Homo sapiens (human)
2	E, F	Methylated-DNA--protein-cysteine methyltransferase,Protein diaphanous homolog 1	polymer	783	86469.3	2	UniProt (P16455) UniProt (O08808) Pfam (PF02870) Pfam (PF01035) Pfam (PF06346) Pfam (PF02181) In PDB	Homo sapiens (human)	6-O-methylguanine-DNA methyltransferase,MGMT,O-6-methylguanine-DNA-alkyltransferase,Diaphanous-related formin-1,DRF1,p140mDIA,mDIA1
3	B, C, D	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	3	Chemie (ADP)
4	B, C, D	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)

Sequence modifications

B, C, D: 2 - 375 (UniProt: P60709)

PDB	External Database	Details
Ala 272	Cys 272	engineered mutation

E, F: 493 - 674 (UniProt: P16455)

PDB	External Database	Details
Met 479	-	initiating methionine
Ala 480	-	expression tag
Ser 481	-	expression tag
Thr 482	-	expression tag
Met 483	-	expression tag
Asp 484	-	expression tag
Ile 485	-	expression tag
Lys 486	-	expression tag
Leu 487	-	expression tag
Thr 488	-	expression tag
Gly 489	-	expression tag
Glu 490	-	expression tag
Phe 491	-	expression tag
Ala 492	-	expression tag
Ala 554	Cys 62	engineered mutation
Ala 617	Lys 125	engineered mutation
Thr 619	Ala 127	engineered mutation
Ala 620	Arg 128	engineered mutation
Lys 623	Gly 131	engineered mutation
Thr 624	Gly 132	engineered mutation
Leu 626	Met 134	engineered mutation
Ser 627	Arg 135	engineered mutation
Ser 642	Cys 150	engineered mutation
Gly 649	Asn 157	engineered mutation
Glu 651	Ser 159	engineered mutation
Pro 675	-	linker
Ala 676	-	linker
Gly 677	-	linker
Gly 678	-	linker
Ser 679	-	linker
Pro 680	-	linker
Gly 681	-	linker
Gly 682	-	linker
Gly 683	-	linker
Ser 684	-	linker
Gly 685	-	linker
Gly 686	-	linker
Ser 687	-	linker

E, F: 688 - 720 (UniProt: O08808)
E, F: 721 - 1255 (UniProt: O08808)

PDB	External Database	Details
His 1256	-	expression tag
His 1257	-	expression tag
His 1258	-	expression tag
His 1259	-	expression tag
His 1260	-	expression tag
His 1261	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	297835.8
Non-Polymers*	Number of molecules	6
	Total formula weight	1354.5
All*	Total formula weight	299190.3

*Water molecules are not included.