8RTY

Structure of the F-actin barbed end bound by Cdc12 and profilin (ring complex) at a resolution of 6.3 Angstrom

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Actin, cytoplasmic 1, N-terminally processed	polymer	374	41632.4	4	UniProt (P60709) Pfam (PF00022) In PDB	Homo sapiens (human)
2	E, F	Methylated-DNA--protein-cysteine methyltransferase,Cell division control protein 12	polymer	703	77888.8	2	UniProt (P16455) UniProt (Q10059) Pfam (PF02870) Pfam (PF01035) Pfam (PF02181) In PDB	Homo sapiens (human)	6-O-methylguanine-DNA methyltransferase,MGMT,O-6-methylguanine-DNA-alkyltransferase
3	H, I, J	Phalloidin (Amanita phalloides)	polymer	7	808.9	3	BIRD (PRD_002366)	Amanita phalloides
4	P	Profilin-1	polymer	139	15000.2	1	UniProt (P07737) Pfam (PF00235) In PDB	Homo sapiens (human)	Epididymis tissue protein Li 184a,Profilin I
5	A, B, C, D	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	4	Chemie (ADP)
6	A, B, C, D	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
7	A, B, C	PHOSPHATE ION	non-polymer		95.0	3	Chemie (PO4)

Sequence modifications

A, B, C, D: 2 - 375 (UniProt: P60709)

PDB	External Database	Details
Ala 272	Cys 272	engineered mutation

E, F: 692 - 872 (UniProt: P16455)

PDB	External Database	Details
Gly 688	-	expression tag
Ala 689	-	expression tag
Met 690	-	expression tag
Ala 691	-	expression tag
Ala 752	Cys 62	engineered mutation
Ala 815	Lys 125	engineered mutation
Thr 817	Ala 127	engineered mutation
Ala 818	Arg 128	engineered mutation
Lys 821	Gly 131	engineered mutation
Thr 822	Gly 132	engineered mutation
Leu 824	Met 134	engineered mutation
Ser 825	Arg 135	engineered mutation
Ser 840	Cys 150	engineered mutation
Gly 847	Asn 157	engineered mutation
Glu 849	Ser 159	engineered mutation
Pro 873	-	linker
Ala 874	-	linker
Gly 875	-	linker
Ile 876	-	linker
Gly 877	-	linker
Ala 878	-	linker
Pro 879	-	linker
Gly 880	-	linker

E, F: 881 - 1390 (UniProt: Q10059)
P: 1 - 139 (UniProt: P07737)

PDB	External Database	Details
Cys 29	Ser 30	engineered mutation
Met 71	Ser 72	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	339734.2
Non-Polymers*	Number of molecules	11
	Total formula weight	2090.9
All*	Total formula weight	341825.2

*Water molecules are not included.