8RGH

Structure of dynein-2 intermediate chain DYNC2I1 (WDR60) in complex with the dynein-2 heavy chain DYNC2H1.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Methylated-DNA--protein-cysteine methyltransferase,Cytoplasmic dynein 2 heavy chain 1	polymer	4513	515223.0	1	UniProt (P16455) UniProt (Q8NCM8) Pfam (PF02870) Pfam (PF12777) Pfam (PF12781) Pfam (PF03028) Pfam (PF18198) Pfam (PF18199) Pfam (PF01035) Pfam (PF08385) Pfam (PF08393) Pfam (PF12774) Pfam (PF21264) Pfam (PF12775) Pfam (PF22597) Pfam (PF12780)	Homo sapiens (human)	6-O-methylguanine-DNA methyltransferase,MGMT,O-6-methylguanine-DNA-alkyltransferase,Cytoplasmic dynein 2 heavy chain,Dynein cytoplasmic heavy chain 2,Dynein heavy chain 11,hDHC11,Dynein heavy chain isotype 1B
2	B (C)	Cytoplasmic dynein 2 intermediate chain 1	polymer	1066	122865.2	1	UniProt (Q8WVS4)	Homo sapiens (human)	Dynein 2 intermediate chain 1,WD repeat-containing protein 60
3	C (D)	Cytoplasmic dynein 2 intermediate chain 2	polymer	564	60639.1	1	UniProt (Q96EX3) Pfam (PF00400)	Homo sapiens (human)	Dynein 2 intermediate chain 2,WD repeat-containing protein 34
4	D (E)	Cytoplasmic dynein 2 light intermediate chain 1	polymer	351	39681.6	1	UniProt (Q8TCX1) Pfam (PF05783)	Homo sapiens (human)	Dynein 2 light intermediate chain
5	E, F (G, H)	Dynein light chain roadblock-type 1	polymer	96	10934.6	2	UniProt (Q9NP97) Pfam (PF03259)	Homo sapiens (human)	Bithoraxoid-like protein,BLP,Dynein light chain 2A,cytoplasmic,Dynein-associated protein Km23,Roadblock domain-containing protein 1

Sequence modifications

A: -204 - -22 (UniProt: P16455)

PDB	External Database	Details
Gly -205	-	expression tag
Arg -176	Glu 30	engineered mutation
Ile -174	Lys 32	engineered mutation
Phe -173	Leu 33	engineered mutation
Ala -144	Cys 62	engineered mutation
Ser -91	Gln 115	engineered mutation
His -90	Gln 116	engineered mutation
Ala -81	Lys 125	engineered mutation
Thr -79	Ala 127	engineered mutation
Ala -78	Arg 128	engineered mutation
Lys -75	Gly 131	engineered mutation
Thr -74	Gly 132	engineered mutation
Leu -72	Met 134	engineered mutation
Ser -71	Arg 135	engineered mutation
Gln -56	Cys 150	engineered mutation
Gly -55	Ser 151	engineered mutation
Asp -54	Ser 152	engineered mutation
Leu -53	Gly 153	engineered mutation
Asp -52	Ala 154	engineered mutation
Gly -49	Asn 157	engineered mutation
Glu -47	Ser 159	engineered mutation
Leu -21	-	linker
Glu -20	-	linker
Val -19	-	linker
Leu -18	-	linker
Phe -17	-	linker
Gln -16	-	linker
Gly -15	-	linker
Pro -14	-	linker
Asp -13	-	linker
Tyr -12	-	linker
Asp -11	-	linker
Ile -10	-	linker
Pro -9	-	linker
Thr -8	-	linker
Thr -7	-	linker
Leu -6	-	linker
Glu -5	-	linker
Val -4	-	linker
Leu -3	-	linker
Phe -2	-	linker
Gln -1	-	linker
Gly 0	-	linker
Pro 1	-	linker

A: 2 - 4307 (UniProt: Q8NCM8)

PDB	External Database	Details
Arg 1413	Lys 1413	variant
Gln 2871	Arg 2871	variant
Val 3680	Ala 3680	variant

C: 1 - 1066 (UniProt: Q8WVS4)

PDB	External Database	Details
Lys 225	Asn 225	conflict
Phe 292	Ser 292	conflict

D: 1 - 536 (UniProt: Q96EX3)

PDB	External Database	Details
Gly 60	Trp 60	conflict
Trp 537	-	expression tag
Ser 538	-	expression tag
His 539	-	expression tag
Pro 540	-	expression tag
Gln 541	-	expression tag
Phe 542	-	expression tag
Glu 543	-	expression tag
Lys 544	-	expression tag
Gly 545	-	expression tag
Ser 546	-	expression tag
Ala 547	-	expression tag
Gly 548	-	expression tag
Ser 549	-	expression tag
Ala 550	-	expression tag
Ala 551	-	expression tag
Gly 552	-	expression tag
Ser 553	-	expression tag
Gly 554	-	expression tag
Ala 555	-	expression tag
Gly 556	-	expression tag
Trp 557	-	expression tag
Ser 558	-	expression tag
His 559	-	expression tag
Pro 560	-	expression tag
Gln 561	-	expression tag
Phe 562	-	expression tag
Glu 563	-	expression tag
Lys 564	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	760278.1
All*	Total formula weight	760278.1

*Water molecules are not included.