8R1G

Dimeric ternary structure of E6AP-E6-p53

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	Ubiquitin-protein ligase E3A	polymer	899	103595.5	2	UniProt (Q05086) Pfam (PF16558) Pfam (PF00632)	Homo sapiens (human)	E6AP ubiquitin-protein ligase,HECT-type ubiquitin transferase E3A,Human papillomavirus E6-associated protein,Oncogenic protein-associated protein E6-AP,Renal carcinoma antigen NY-REN-54
2	B, E (B, E)	Ubiquitin-like protein SMT3,Protein E6	polymer	266	31543.0	2	UniProt (Q12306) UniProt (P03126) Pfam (PF00240) Pfam (PF00518)	Saccharomyces cerevisiae S288C
3	C, F (C, F)	Cellular tumor antigen p53	polymer	417	46495.2	2	UniProt (P04637) Pfam (PF08563) Pfam (PF18521) Pfam (PF00870) Pfam (PF07710)	Homo sapiens (human)	Antigen NY-CO-13,Phosphoprotein p53,Tumor suppressor p53
4	G, H, I, J, K... (B, C, E, F)	ZINC ION	non-polymer		65.4	6	Chemie (ZN)

Sequence modifications

A, D: 1 - 875 (UniProt: Q05086)

PDB	External Database	Details
Met -23	-	initiating methionine
Asp -22	-	expression tag
Trp -21	-	expression tag
Ser -20	-	expression tag
His -19	-	expression tag
Pro -18	-	expression tag
Gln -17	-	expression tag
Phe -16	-	expression tag
Glu -15	-	expression tag
Lys -14	-	expression tag
Ser -13	-	expression tag
Ala -12	-	expression tag
Val -11	-	expression tag
Asp -10	-	expression tag
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

B, E: -107 - -10 (UniProt: Q12306)

PDB	External Database	Details
Ser -9	-	linker
Ala -8	-	linker
Asp -7	-	linker
Glu -6	-	linker
Asn -5	-	linker
Leu -4	-	linker
Tyr -3	-	linker
Phe -2	-	linker
Gln -1	-	linker
Gly 0	-	linker

B, E: 1 - 158 (UniProt: P03126)

PDB	External Database	Details
Ser 87	Cys 87	engineered mutation
Ser 104	Cys 104	engineered mutation
Ser 118	Cys 118	engineered mutation
Ser 147	Cys 147	engineered mutation

C, F: 1 - 393 (UniProt: P04637)

PDB	External Database	Details
Met -23	-	initiating methionine
Asp -22	-	expression tag
Trp -21	-	expression tag
Ser -20	-	expression tag
His -19	-	expression tag
Pro -18	-	expression tag
Gln -17	-	expression tag
Phe -16	-	expression tag
Glu -15	-	expression tag
Lys -14	-	expression tag
Ser -13	-	expression tag
Ala -12	-	expression tag
Val -11	-	expression tag
Asp -10	-	expression tag
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	363267.3
Non-Polymers*	Number of molecules	6
	Total formula weight	392.5
All*	Total formula weight	363659.7

*Water molecules are not included.