8R0J

Crystal structure of the retromer complex VPS29/VPS35 with the ligand bis-1,3-phenyl guanylhydrazone, 2a

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Vacuolar protein sorting-associated protein 29	polymer	185	20930.2	2	UniProt (Q9UBQ0) Pfam (PF12850) In PDB	Homo sapiens (human)
2	C, D	Vacuolar protein sorting-associated protein 35	polymer	306	35243.1	2	UniProt (Q96QK1) Pfam (PF03635) In PDB	Homo sapiens (human)
3	C	Bis-1,3-phenyl guanylhydrazon	non-polymer		246.3	1	Chemie (XFZ)
4	D	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
5		water	water		18.0	417	Chemie (HOH)

Sequence modifications

A, B: 1 - 182 (UniProt: Q9UBQ0)

PDB	External Database	Details
Met -2	-	initiating methionine
Glu -1	-	expression tag
His 0	-	expression tag

C, D: 476 - 780 (UniProt: Q96QK1)

PDB	External Database	Details
Met 475	-	initiating methionine

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	112346.5
Non-Polymers*	Number of molecules	2
	Total formula weight	368.4
All*	Total formula weight	112714.9

*Water molecules are not included.