8R0J
Crystal structure of the retromer complex VPS29/VPS35 with the ligand bis-1,3-phenyl guanylhydrazone, 2a
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-30 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.87313 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.150, 130.340, 146.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.746 - 2.400 |
Rwork | 0.185 |
R-free | 0.24980 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.409 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.746 | 2.450 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmeas | 0.398 | |
Number of reflections | 37129 | 2689 |
<I/σ(I)> | 6.5 | 1.1 |
Completeness [%] | 99.9 | |
Redundancy | 12.8 | |
CC(1/2) | 0.992 | 0.506 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 18% PEG 3350, 150 mM NaK tartrate, pH 7.4; |