8QZ3
Crystal structure of human two pore domain potassium ion channel TREK-2 (K2P10.1) in complex with an activatory nanobody (Nb67)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Potassium channel subfamily K member 10 | polymer | 274 | 30157.0 | 2 | UniProt (P57789) Pfam (PF00520) Pfam (PF07885) | Homo sapiens (human) | Outward rectifying potassium channel protein TREK-2,TREK-2 K(+) channel subunit |
| 2 | C, D, E (C, D, E) | Nanobody 67 | polymer | 137 | 14944.4 | 3 | Lama glama | ||
| 3 | F, G, H, I, J (A, B) | POTASSIUM ION | non-polymer | 39.1 | 5 | Chemie (K) | |||
| 4 | K (D) | (4S)-2-METHYL-2,4-PENTANEDIOL | non-polymer | 118.2 | 1 | Chemie (MPD) | |||
| 5 | L, M, N, O, P (A, B, C, D, E) | water | water | 18.0 | 191 | Chemie (HOH) |
Sequence modifications
A, B: 75 - 340 (UniProt: P57789)
| PDB | External Database | Details |
|---|---|---|
| Met 74 | - | initiating methionine |
| Gln 149 | Asn 149 | engineered mutation |
| Gln 152 | Asn 152 | engineered mutation |
| Gln 153 | Asn 153 | engineered mutation |
| Ala 341 | - | expression tag |
| Glu 342 | - | expression tag |
| Asn 343 | - | expression tag |
| Leu 344 | - | expression tag |
| Tyr 345 | - | expression tag |
| Phe 346 | - | expression tag |
| Gln 347 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 105147.2 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 313.7 | |
| All* | Total formula weight | 105460.8 |
