8QZ3
Crystal structure of human two pore domain potassium ion channel TREK-2 (K2P10.1) in complex with an activatory nanobody (Nb67)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 51.264, 102.100, 215.817 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.810 - 2.400 |
| R-factor | 0.2782 |
| Rwork | 0.278 |
| R-free | 0.29090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.680 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.808 | 2.492 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmeas | 0.219 | 1.363 |
| Rpim | 0.087 | 0.536 |
| Number of reflections | 34388 | 1719 |
| <I/σ(I)> | 6.3 | 1.6 |
| Completeness [%] | 75.6 | 35.9 |
| Redundancy | 6.4 | 6.3 |
| CC(1/2) | 0.989 | 0.610 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.1 M Tris pH 8.0, 65% v/v 2-methyl-2,4-pentanediol |
