8QU8
PROTAC-mediated complex of KRAS with VHL/Elongin-B/Elongin-C/Cullin-2/Rbx1
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | von Hippel-Lindau disease tumor suppressor | polymer | 212 | 24049.5 | 1 | UniProt (P40337) Pfam (PF01847) Pfam (PF17211) | Homo sapiens (human) | |
| 2 | B (B) | Elongin-B | polymer | 117 | 13016.6 | 1 | UniProt (Q15370) Pfam (PF00240) | Homo sapiens (human) | EloB,Elongin 18 kDa subunit,RNA polymerase II transcription factor SIII subunit B,SIII p18,Transcription elongation factor B polypeptide 2 |
| 3 | C (C) | Elongin-C | polymer | 111 | 12353.9 | 1 | UniProt (Q15369) Pfam (PF03931) | Homo sapiens (human) | EloC,Elongin 15 kDa subunit,RNA polymerase II transcription factor SIII subunit C,SIII p15,Transcription elongation factor B polypeptide 1 |
| 4 | D (D) | Cullin-2 | polymer | 744 | 86967.7 | 1 | UniProt (Q13617) Pfam (PF00888) Pfam (PF26557) Pfam (PF10557) | Homo sapiens (human) | CUL-2 |
| 5 | E (E) | E3 ubiquitin-protein ligase RBX1, N-terminally processed | polymer | 107 | 12158.8 | 1 | UniProt (P62877) Pfam (PF12678) | Homo sapiens (human) | E3 ubiquitin-protein transferase RBX1,N-terminally processed |
| 6 | F (F) | GTPase KRas | polymer | 170 | 19354.8 | 1 | UniProt (P01116) Pfam (PF00071) | Homo sapiens (human) | K-Ras 2,Ki-Ras,c-K-ras,c-Ki-ras |
| 7 | G, H (E) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 8 | I (F) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537) | |||
| 9 | J (F) | (2S,4R)-1-[(2S)-2-[4-[4-[(3S)-4-[4-[5-[(4S)-2-azanyl-3-cyano-4-methyl-6,7-dihydro-5H-1-benzothiophen-4-yl]-1,2,4-oxadiazol-3-yl]pyrimidin-2-yl]-3-methyl-1,4-diazepan-1-yl]butoxy]-1,2,3-triazol-1-yl]-3-methyl-butanoyl]-N-[(1R)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]-2-oxidanyl-ethyl]-4-oxidanyl-pyrrolidine-2-carboxamide | non-polymer | 1019.2 | 1 | Chemie (WYL) PubChem (169410198) PubChem (169449364) PubChem (172875658) PubChem (172875721) |
Sequence modifications
F: 1 - 164 (UniProt: P01116)
| PDB | External Database | Details |
|---|---|---|
| Gly 0 | - | expression tag |
| Val 12 | Gly 12 | engineered mutation |
| Ser 118 | Cys 118 | engineered mutation |
| Gly 151 | Arg 151 | variant |
| Asp 153 | Glu 153 | variant |
| Lys 165 | - | expression tag |
| His 166 | - | expression tag |
| Lys 167 | - | expression tag |
| Glu 168 | - | expression tag |
| Lys 169 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 167901.4 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1593.3 | |
| All* | Total formula weight | 169494.7 |
