8QU8

PROTAC-mediated complex of KRAS with VHL/Elongin-B/Elongin-C/Cullin-2/Rbx1

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000122	biological_process	negative regulation of transcription by RNA polymerase II
A	0000902	biological_process	cell morphogenesis
A	0003711	molecular_function	transcription elongation factor activity
A	0003714	molecular_function	transcription corepressor activity
A	0004842	molecular_function	ubiquitin-protein transferase activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005783	cellular_component	endoplasmic reticulum
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0006355	biological_process	regulation of DNA-templated transcription
A	0006508	biological_process	proteolysis
A	0008285	biological_process	negative regulation of cell population proliferation
A	0009968	biological_process	negative regulation of signal transduction
A	0010468	biological_process	regulation of gene expression
A	0010507	biological_process	negative regulation of autophagy
A	0010629	biological_process	negative regulation of gene expression
A	0016567	biological_process	protein ubiquitination
A	0019899	molecular_function	enzyme binding
A	0034244	biological_process	negative regulation of transcription elongation by RNA polymerase II
A	0043066	biological_process	negative regulation of apoptotic process
A	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
A	0043232	cellular_component	intracellular non-membrane-bounded organelle
A	0045597	biological_process	positive regulation of cell differentiation
A	0045893	biological_process	positive regulation of DNA-templated transcription
A	0046426	biological_process	negative regulation of receptor signaling pathway via JAK-STAT
A	0050821	biological_process	protein stabilization
A	0060090	molecular_function	molecular adaptor activity
A	0071456	biological_process	cellular response to hypoxia
A	0140297	molecular_function	DNA-binding transcription factor binding
A	1900037	biological_process	regulation of cellular response to hypoxia
A	1904262	biological_process	negative regulation of TORC1 signaling
A	1990000	biological_process	amyloid fibril formation
A	1990756	molecular_function	ubiquitin-like ligase-substrate adaptor activity
B	0001222	molecular_function	transcription corepressor binding
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005829	cellular_component	cytosol
B	0006367	biological_process	transcription initiation at RNA polymerase II promoter
B	0006368	biological_process	transcription elongation by RNA polymerase II
B	0016567	biological_process	protein ubiquitination
B	0030891	cellular_component	VCB complex
B	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
B	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
B	0031625	molecular_function	ubiquitin protein ligase binding
B	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
B	0065003	biological_process	protein-containing complex assembly
B	0070449	cellular_component	elongin complex
B	0140958	biological_process	target-directed miRNA degradation
C	0001222	molecular_function	transcription corepressor binding
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005829	cellular_component	cytosol
C	0006357	biological_process	regulation of transcription by RNA polymerase II
C	0006367	biological_process	transcription initiation at RNA polymerase II promoter
C	0006511	biological_process	ubiquitin-dependent protein catabolic process
C	0016567	biological_process	protein ubiquitination
C	0030674	molecular_function	protein-macromolecule adaptor activity
C	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
C	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
C	0070449	cellular_component	elongin complex
C	0140958	biological_process	target-directed miRNA degradation
D	0000082	biological_process	G1/S transition of mitotic cell cycle
D	0004842	molecular_function	ubiquitin-protein transferase activity
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005730	cellular_component	nucleolus
D	0005829	cellular_component	cytosol
D	0006511	biological_process	ubiquitin-dependent protein catabolic process
D	0016567	biological_process	protein ubiquitination
D	0019005	cellular_component	SCF ubiquitin ligase complex
D	0030163	biological_process	protein catabolic process
D	0030674	molecular_function	protein-macromolecule adaptor activity
D	0030891	cellular_component	VCB complex
D	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
D	0031461	cellular_component	cullin-RING ubiquitin ligase complex
D	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
D	0031625	molecular_function	ubiquitin protein ligase binding
D	0044877	molecular_function	protein-containing complex binding
D	0097193	biological_process	intrinsic apoptotic signaling pathway
D	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
D	0160072	molecular_function	ubiquitin ligase complex scaffold activity
E	0000165	biological_process	MAPK cascade
E	0000209	biological_process	protein polyubiquitination
E	0004842	molecular_function	ubiquitin-protein transferase activity
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006281	biological_process	DNA repair
E	0006511	biological_process	ubiquitin-dependent protein catabolic process
E	0006513	biological_process	protein monoubiquitination
E	0006974	biological_process	DNA damage response
E	0007283	biological_process	spermatogenesis
E	0008270	molecular_function	zinc ion binding
E	0016567	biological_process	protein ubiquitination
E	0016740	molecular_function	transferase activity
E	0019005	cellular_component	SCF ubiquitin ligase complex
E	0019788	molecular_function	NEDD8 transferase activity
E	0030163	biological_process	protein catabolic process
E	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
E	0031461	cellular_component	cullin-RING ubiquitin ligase complex
E	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
E	0031463	cellular_component	Cul3-RING ubiquitin ligase complex
E	0031464	cellular_component	Cul4A-RING E3 ubiquitin ligase complex
E	0031465	cellular_component	Cul4B-RING E3 ubiquitin ligase complex
E	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
E	0031467	cellular_component	Cul7-RING ubiquitin ligase complex
E	0031625	molecular_function	ubiquitin protein ligase binding
E	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
E	0032480	biological_process	negative regulation of type I interferon production
E	0034450	molecular_function	ubiquitin-ubiquitin ligase activity
E	0034644	biological_process	cellular response to UV
E	0042110	biological_process	T cell activation
E	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
E	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
E	0043687	biological_process	post-translational protein modification
E	0045116	biological_process	protein neddylation
E	0045732	biological_process	positive regulation of protein catabolic process
E	0046872	molecular_function	metal ion binding
E	0060090	molecular_function	molecular adaptor activity
E	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
E	0061630	molecular_function	ubiquitin protein ligase activity
E	0061663	molecular_function	NEDD8 ligase activity
E	0062197	biological_process	cellular response to chemical stress
E	0070936	biological_process	protein K48-linked ubiquitination
E	0071230	biological_process	cellular response to amino acid stimulus
E	0090090	biological_process	negative regulation of canonical Wnt signaling pathway
E	0097602	molecular_function	cullin family protein binding
E	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
E	1900076	biological_process	regulation of cellular response to insulin stimulus
E	1902499	biological_process	positive regulation of protein autoubiquitination
E	1902883	biological_process	negative regulation of response to oxidative stress
E	1904263	biological_process	positive regulation of TORC1 signaling
F	0003924	molecular_function	GTPase activity
F	0005525	molecular_function	GTP binding
F	0007165	biological_process	signal transduction
F	0016020	cellular_component	membrane

Functional Information from PROSITE/UniProt

site_id	PS01256
Number of Residues	28
Details	CULLIN_1 Cullin family signature. IKkcIevLIDKqYIeRsqasadeYsYvA

Chain	Residue	Details
D	ILE718-ALA745

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:22431598, ECO:0000269|PubMed:22566140

Chain	Residue	Details
F	GLY10
F	VAL29
F	ALA59
F	ASN116

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: N-acetylmethionine; in GTPase KRas; alternate => ECO:0000269|Ref.17

Chain	Residue	Details
F	MET1
E	CYS94
E	ASP97
E	CYS45
E	CYS53
E	CYS56
E	CYS68
E	CYS75
E	HIS77
E	HIS80
E	HIS82

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: N-acetylthreonine; in GTPase KRas, N-terminally processed => ECO:0000269|Ref.17

Chain	Residue	Details
F	THR2

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: N6-acetyllysine => ECO:0000269|PubMed:22711838

Chain	Residue	Details
F	LYS104

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	CARBOHYD: (Microbial infection) O-linked (Glc) threonine; by P.sordellii toxin TcsL => ECO:0000269|PubMed:19744486

Chain	Residue	Details
F	THR35

---