8QU8

PROTAC-mediated complex of KRAS with VHL/Elongin-B/Elongin-C/Cullin-2/Rbx1

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000122	biological_process	negative regulation of transcription by RNA polymerase II
A	0000902	biological_process	cell morphogenesis
A	0001666	biological_process	response to hypoxia
A	0003711	molecular_function	transcription elongation factor activity
A	0003714	molecular_function	transcription corepressor activity
A	0004842	molecular_function	ubiquitin-protein transferase activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005783	cellular_component	endoplasmic reticulum
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0006355	biological_process	regulation of DNA-templated transcription
A	0006508	biological_process	proteolysis
A	0008285	biological_process	negative regulation of cell population proliferation
A	0009968	biological_process	negative regulation of signal transduction
A	0010468	biological_process	regulation of gene expression
A	0010507	biological_process	negative regulation of autophagy
A	0010629	biological_process	negative regulation of gene expression
A	0016567	biological_process	protein ubiquitination
A	0019899	molecular_function	enzyme binding
A	0034244	biological_process	negative regulation of transcription elongation by RNA polymerase II
A	0043066	biological_process	negative regulation of apoptotic process
A	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
A	0045597	biological_process	positive regulation of cell differentiation
A	0045893	biological_process	positive regulation of DNA-templated transcription
A	0046426	biological_process	negative regulation of receptor signaling pathway via JAK-STAT
A	0050821	biological_process	protein stabilization
A	0060090	molecular_function	molecular adaptor activity
A	0071456	biological_process	cellular response to hypoxia
A	0120283	molecular_function	protein serine/threonine kinase binding
A	0140297	molecular_function	DNA-binding transcription factor binding
A	1900037	biological_process	regulation of cellular response to hypoxia
A	1904262	biological_process	negative regulation of TORC1 signaling
A	1990000	biological_process	amyloid fibril formation
A	1990756	molecular_function	ubiquitin-like ligase-substrate adaptor activity
B	0000122	biological_process	negative regulation of transcription by RNA polymerase II
B	0000151	cellular_component	ubiquitin ligase complex
B	0001222	molecular_function	transcription corepressor binding
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006351	biological_process	DNA-templated transcription
B	0006367	biological_process	transcription initiation at RNA polymerase II promoter
B	0006368	biological_process	transcription elongation by RNA polymerase II
B	0016567	biological_process	protein ubiquitination
B	0030891	cellular_component	VCB complex
B	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
B	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
B	0031625	molecular_function	ubiquitin protein ligase binding
B	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
B	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
B	0065003	biological_process	protein-containing complex assembly
B	0070449	cellular_component	elongin complex
B	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
B	0140958	biological_process	target-directed miRNA degradation
B	1990116	biological_process	ribosome-associated ubiquitin-dependent protein catabolic process
B	2000104	biological_process	negative regulation of DNA-templated DNA replication
C	0000122	biological_process	negative regulation of transcription by RNA polymerase II
C	0000151	cellular_component	ubiquitin ligase complex
C	0001222	molecular_function	transcription corepressor binding
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006351	biological_process	DNA-templated transcription
C	0006357	biological_process	regulation of transcription by RNA polymerase II
C	0006367	biological_process	transcription initiation at RNA polymerase II promoter
C	0006511	biological_process	ubiquitin-dependent protein catabolic process
C	0016567	biological_process	protein ubiquitination
C	0030674	molecular_function	protein-macromolecule adaptor activity
C	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
C	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
C	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
C	0070449	cellular_component	elongin complex
C	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
C	0140958	biological_process	target-directed miRNA degradation
C	1990116	biological_process	ribosome-associated ubiquitin-dependent protein catabolic process
C	2000104	biological_process	negative regulation of DNA-templated DNA replication
D	0000082	biological_process	G1/S transition of mitotic cell cycle
D	0000122	biological_process	negative regulation of transcription by RNA polymerase II
D	0004842	molecular_function	ubiquitin-protein transferase activity
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005730	cellular_component	nucleolus
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006511	biological_process	ubiquitin-dependent protein catabolic process
D	0010498	biological_process	proteasomal protein catabolic process
D	0016567	biological_process	protein ubiquitination
D	0019005	cellular_component	SCF ubiquitin ligase complex
D	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
D	0031461	cellular_component	cullin-RING ubiquitin ligase complex
D	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
D	0031625	molecular_function	ubiquitin protein ligase binding
D	0031981	cellular_component	nuclear lumen
D	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
D	0072344	biological_process	rescue of stalled ribosome
D	0097193	biological_process	intrinsic apoptotic signaling pathway
D	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
D	0160072	molecular_function	ubiquitin ligase complex scaffold activity
D	0160276	biological_process	negative regulation of beige fat cell differentiation
D	1990116	biological_process	ribosome-associated ubiquitin-dependent protein catabolic process
D	2000104	biological_process	negative regulation of DNA-templated DNA replication
E	0000082	biological_process	G1/S transition of mitotic cell cycle
E	0000122	biological_process	negative regulation of transcription by RNA polymerase II
E	0000165	biological_process	MAPK cascade
E	0000209	biological_process	protein polyubiquitination
E	0004842	molecular_function	ubiquitin-protein transferase activity
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005737	cellular_component	cytoplasm
E	0005794	cellular_component	Golgi apparatus
E	0005813	cellular_component	centrosome
E	0005829	cellular_component	cytosol
E	0006281	biological_process	DNA repair
E	0006283	biological_process	transcription-coupled nucleotide-excision repair
E	0006289	biological_process	nucleotide-excision repair
E	0006355	biological_process	regulation of DNA-templated transcription
E	0006511	biological_process	ubiquitin-dependent protein catabolic process
E	0006513	biological_process	protein monoubiquitination
E	0006879	biological_process	intracellular iron ion homeostasis
E	0006974	biological_process	DNA damage response
E	0007283	biological_process	spermatogenesis
E	0007346	biological_process	regulation of mitotic cell cycle
E	0008270	molecular_function	zinc ion binding
E	0008283	biological_process	cell population proliferation
E	0010506	biological_process	regulation of autophagy
E	0010564	biological_process	regulation of cell cycle process
E	0010824	biological_process	regulation of centrosome duplication
E	0014033	biological_process	neural crest cell differentiation
E	0016567	biological_process	protein ubiquitination
E	0016740	molecular_function	transferase activity
E	0019005	cellular_component	SCF ubiquitin ligase complex
E	0019788	molecular_function	NEDD8 transferase activity
E	0030174	biological_process	regulation of DNA-templated DNA replication initiation
E	0030510	biological_process	regulation of BMP signaling pathway
E	0030891	cellular_component	VCB complex
E	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
E	0031297	biological_process	replication fork processing
E	0031461	cellular_component	cullin-RING ubiquitin ligase complex
E	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
E	0031463	cellular_component	Cul3-RING ubiquitin ligase complex
E	0031464	cellular_component	Cul4A-RING E3 ubiquitin ligase complex
E	0031465	cellular_component	Cul4B-RING E3 ubiquitin ligase complex
E	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
E	0031467	cellular_component	Cul7-RING ubiquitin ligase complex
E	0031625	molecular_function	ubiquitin protein ligase binding
E	0032006	biological_process	regulation of TOR signaling
E	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
E	0032480	biological_process	negative regulation of type I interferon production
E	0032814	biological_process	regulation of natural killer cell activation
E	0034450	molecular_function	ubiquitin-ubiquitin ligase activity
E	0034599	biological_process	cellular response to oxidative stress
E	0034644	biological_process	cellular response to UV
E	0040029	biological_process	epigenetic regulation of gene expression
E	0042110	biological_process	T cell activation
E	0042127	biological_process	regulation of cell population proliferation
E	0042752	biological_process	regulation of circadian rhythm
E	0042770	biological_process	signal transduction in response to DNA damage
E	0042981	biological_process	regulation of apoptotic process
E	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
E	0043124	biological_process	negative regulation of canonical NF-kappaB signal transduction
E	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
E	0043687	biological_process	post-translational protein modification
E	0044314	biological_process	protein K27-linked ubiquitination
E	0044877	molecular_function	protein-containing complex binding
E	0045116	biological_process	protein neddylation
E	0045732	biological_process	positive regulation of protein catabolic process
E	0045995	biological_process	regulation of embryonic development
E	0046627	biological_process	negative regulation of insulin receptor signaling pathway
E	0046872	molecular_function	metal ion binding
E	0050727	biological_process	regulation of inflammatory response
E	0051298	biological_process	centrosome duplication
E	0051726	biological_process	regulation of cell cycle
E	0060090	molecular_function	molecular adaptor activity
E	0060173	biological_process	limb development
E	0060271	biological_process	cilium assembly
E	0060964	biological_process	regulation of miRNA-mediated gene silencing
E	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
E	0061630	molecular_function	ubiquitin protein ligase activity
E	0061663	molecular_function	NEDD8 ligase activity
E	0062197	biological_process	cellular response to chemical stress
E	0070936	biological_process	protein K48-linked ubiquitination
E	0071230	biological_process	cellular response to amino acid stimulus
E	0080008	cellular_component	Cul4-RING E3 ubiquitin ligase complex
E	0080135	biological_process	regulation of cellular response to stress
E	0090090	biological_process	negative regulation of canonical Wnt signaling pathway
E	0090734	cellular_component	site of DNA damage
E	0097602	molecular_function	cullin family protein binding
E	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
E	0160240	biological_process	RNA polymerase II transcription initiation surveillance
E	0160276	biological_process	negative regulation of beige fat cell differentiation
E	1900076	biological_process	regulation of cellular response to insulin stimulus
E	1901524	biological_process	regulation of mitophagy
E	1901525	biological_process	negative regulation of mitophagy
E	1901987	biological_process	regulation of cell cycle phase transition
E	1902412	biological_process	regulation of mitotic cytokinesis
E	1902499	biological_process	positive regulation of protein autoubiquitination
E	1902883	biological_process	negative regulation of response to oxidative stress
E	1904178	biological_process	negative regulation of adipose tissue development
E	1904263	biological_process	positive regulation of TORC1 signaling
E	1904415	biological_process	regulation of xenophagy
E	1990116	biological_process	ribosome-associated ubiquitin-dependent protein catabolic process
E	2000001	biological_process	regulation of DNA damage checkpoint
E	2000036	biological_process	regulation of stem cell population maintenance
E	2000104	biological_process	negative regulation of DNA-templated DNA replication
F	0003924	molecular_function	GTPase activity
F	0005525	molecular_function	GTP binding
F	0007165	biological_process	signal transduction
F	0016020	cellular_component	membrane

Functional Information from PROSITE/UniProt

site_id	PS01256
Number of Residues	28
Details	CULLIN_1 Cullin family signature. IKkcIevLIDKqYIeRsqasadeYsYvA

Chain	Residue	Details
D	ILE718-ALA745

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	55
Details	Region: {"description":"Involved in binding to CCT complex"}

Chain

Residue

Details

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site_id	SWS_FT_FI2
Number of Residues	9
Details	Region: {"description":"Interaction with Elongin BC complex"}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"UniProtKB","id":"P62869","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	60
Details	Domain: {"description":"Cullin neddylation","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"18669648","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in NEDD8)","evidences":[{"source":"PubMed","id":"10092517","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"10597293","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	45
Details	Zinc finger: {"description":"RING-type","evidences":[{"source":"PROSITE-ProRule","id":"PRU00175","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	7
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11961546","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38605244","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1LDJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1LDK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1U6G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2HYE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3DPL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3DQV","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3RTR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4F52","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4P5O","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Q7H","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8RHZ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11961546","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38605244","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1LDJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1LDK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1U6G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2HYE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3DPL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3DQV","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3RTR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4F52","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4P5O","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7Z8B","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Q7H","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8RHZ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11961546","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38605244","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1LDJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1LDK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1U6G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2HYE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3DQV","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3RTR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4F52","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4P5O","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Q7H","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8RHZ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	8
Details	Motif: {"description":"Effector region"}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	18
Details	Binding site: {"evidences":[{"source":"PubMed","id":"22431598","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"22566140","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"34380736","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"35522713","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	1
Details	Modified residue: {"description":"N-acetylmethionine; in GTPase KRas; alternate","evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"FEB-2008","submissionDatabase":"UniProtKB","authors":["Bienvenut W.V.","Calvo F.","Kolch W."]}}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	1
Details	Modified residue: {"description":"N-acetylthreonine; in GTPase KRas, N-terminally processed","evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"FEB-2008","submissionDatabase":"UniProtKB","authors":["Bienvenut W.V.","Calvo F.","Kolch W."]}}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	1
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"22711838","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI17
Number of Residues	1
Details	Glycosylation: {"description":"(Microbial infection) O-linked (Glc) threonine; by P.sordellii toxin TcsL","evidences":[{"source":"PubMed","id":"19744486","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---