8QEZ
Crystal structure of the AMPA receptor GluA2-L504Y-N775S ligand binding domain in complex with L-glutamate and positive allosteric modulator BPAM395 at 1.55A resolution
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (A, B, C) | Glutamate receptor 2 | polymer | 264 | 29301.7 | 3 | UniProt (P19491) Pfam (PF10613) Pfam (PF00060) | Rattus norvegicus (Norway rat) | GluR-2,AMPA-selective glutamate receptor 2,GluR-B,GluR-K2,Glutamate receptor ionotropic,AMPA 2,GluA2 |
| 2 | D, O, W (A, B, C) | GLUTAMIC ACID | non-polymer | 147.1 | 3 | Chemie (GLU) | |||
| 3 | E, F, G, H, P... (A, B, C) | ZINC ION | non-polymer | 65.4 | 7 | Chemie (ZN) | |||
| 4 | AA, I, J, K, L... (C, A, B) | ACETATE ION | non-polymer | 59.0 | 8 | Chemie (ACT) | |||
| 5 | EA, M, U (C, A, B) | 6-chloranyl-4-cyclopropyl-2,3-dihydrothieno[3,2-e][1,2,4]thiadiazine 1,1-dioxide | non-polymer | 264.8 | 3 | Chemie (UF5) | |||
| 6 | FA, N, V (C, A, B) | CHLORIDE ION | non-polymer | 35.5 | 3 | Chemie (CL) | |||
| 7 | BA, CA, DA, T (C, B) | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
| 8 | Z (C) | CACODYLATE ION | non-polymer | 137.0 | 1 | Chemie (CAC) | |||
| 9 | GA, HA, IA (A, B, C) | water | water | 18.0 | 789 | Chemie (HOH) |
Sequence modifications
A, B, C: 3 - 117 (UniProt: P19491)
A, B, C: 120 - 264 (UniProt: P19491)
| PDB | External Database | Details |
|---|---|---|
| Gly 1 | - | expression tag |
| Ala 2 | - | expression tag |
| Tyr 94 | Leu 504 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 118 | - | linker |
| Thr 119 | - | linker |
| Ser 242 | Asn 775 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 87905.2 | |
| Non-Polymers* | Number of molecules | 29 |
| Total formula weight | 2777.6 | |
| All* | Total formula weight | 90682.8 |
