8QEZ

Crystal structure of the AMPA receptor GluA2-L504Y-N775S ligand binding domain in complex with L-glutamate and positive allosteric modulator BPAM395 at 1.55A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-06
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97625
Spacegroup name	P 21 21 2
Unit cell lengths	114.316, 163.416, 47.466
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.170 - 1.550
R-factor	0.169
Rwork	0.168
R-free	0.19520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.885
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.20_4459: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.170	1.630
High resolution limit [Å]	1.550	1.550
Rmerge	0.071	0.879
Rmeas	0.077	0.953
Rpim	0.029	0.367
Total number of observations	864426	124663
Number of reflections	129920	18786
<I/σ(I)>	13.1	2
Completeness [%]	100.0
Redundancy	6.7	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	279	15.2% PEG4000, 0.1M Zn Acetate, 0.1M Cacodylate pH 6.5