8QEZ
Crystal structure of the AMPA receptor GluA2-L504Y-N775S ligand binding domain in complex with L-glutamate and positive allosteric modulator BPAM395 at 1.55A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-06 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 114.316, 163.416, 47.466 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.170 - 1.550 |
| R-factor | 0.169 |
| Rwork | 0.168 |
| R-free | 0.19520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.885 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20_4459: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.170 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.071 | 0.879 |
| Rmeas | 0.077 | 0.953 |
| Rpim | 0.029 | 0.367 |
| Total number of observations | 864426 | 124663 |
| Number of reflections | 129920 | 18786 |
| <I/σ(I)> | 13.1 | 2 |
| Completeness [%] | 100.0 | |
| Redundancy | 6.7 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 279 | 15.2% PEG4000, 0.1M Zn Acetate, 0.1M Cacodylate pH 6.5 |
