8Q5U
Endoglycosidase S2 in complex with IgG1 Fc
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, C, B | Uncharacterized protein DKFZp686C11235 | polymer | 227 | 25545.0 | 3 | UniProt (Q6MZV7) Pfam (PF07654) UniProt (by SIFTS) (P0DOX5) In PDB | Homo sapiens (human) | |
2 | D, E, F | Endo-beta-N-acetylglucosaminidase | polymer | 816 | 92581.2 | 3 | UniProt (A0A8H2N1T2) Pfam (PF20746) UniProt (by SIFTS) (T1WGN1) In PDB | Streptococcus pyogenes | |
3 | G | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1463.3 | 1 | In PDB GlyTouCan (G80858MF) | |||
4 | H | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1260.2 | 1 | In PDB GlyTouCan (G45889JQ) | |||
5 | A, D, B | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
6 | D, F | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 5 | Chemie (PGE) | |||
7 | D, E, F | CALCIUM ION | non-polymer | 40.1 | 3 | Chemie (CA) | |||
8 | water | water | 18.0 | 281 | Chemie (HOH) |
Sequence modifications
A, C, B: 221 - 447 (UniProt: Q6MZV7)
D, E, F: 38 - 843 (UniProt: A0A8H2N1T2)
PDB | External Database | Details |
---|---|---|
Cys 234 | Leu 260 | engineered mutation |
Ala 382 | Glu 408 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Met 37 | - | initiating methionine |
Ala 184 | Asp 184 | engineered mutation |
Leu 186 | Glu 186 | engineered mutation |
Leu 844 | - | expression tag |
Leu 845 | - | expression tag |
Glu 846 | - | expression tag |
His 847 | - | expression tag |
His 848 | - | expression tag |
His 849 | - | expression tag |
His 850 | - | expression tag |
His 851 | - | expression tag |
His 852 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 354378.6 | |
Branched | Number of molecules | 2 |
Total formula weight | 2723.5 | |
Non-Polymers* | Number of molecules | 12 |
Total formula weight | 1239.5 | |
All* | Total formula weight | 358341.6 |