8Q4D

IstA-IstB(E167Q) Strand Transfer Complex

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Putative transposase for insertion sequence element IS5376	polymer	373	44012.7	4	UniProt (Q45618) In PDB	Geobacillus stearothermophilus
2	E, F, G, H, I...	Insertion sequence IS5376 putative ATP-binding protein	polymer	247	28817.4	20	UniProt (Q45619) In PDB	Geobacillus stearothermophilus
3	a, d	DNA (118-MER) / TIR-transferred strand	polymer	118	36524.4	2	GenBank (1801971458)	Geobacillus stearothermophilus
4	b, e	DNA (58-MER) / TIR non-transferred strand	polymer	58	17767.4	2	GenBank (1190175513)	Geobacillus stearothermophilus
5	c, f	DNA (58-MER) / target-reverse complement	polymer	58	17836.4	2	GenBank (1801971458)	Geobacillus stearothermophilus
6	P, Q, A, R, B...	MAGNESIUM ION	non-polymer		24.3	20	Chemie (MG)
7	E, O	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)
8	P, Q, R, S, F...	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	18	Chemie (ATP)

Sequence modifications

E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X: 1 - 246 (UniProt: Q45619)

PDB	External Database	Details
Asn 0	-	expression tag
Gln 167	Glu 167	engineered mutation

a, d: 61 - 118 (GenBank: 1801971458)

PDB	External Database	Details
Dc 1	-	expression tag
Dc 2	-	expression tag
Dt 3	-	expression tag
Dt 4	-	expression tag
Dc 5	-	expression tag
Dt 6	-	expression tag
Dg 7	-	expression tag
Dg 8	-	expression tag
Dg 9	-	expression tag
Dg 10	-	expression tag
Da 11	-	expression tag
Da 12	-	expression tag
Dt 13	-	expression tag
Dt 14	-	expression tag
Dt 15	-	expression tag
Dt 16	-	expression tag
Da 17	-	expression tag
Da 18	-	expression tag
Da 19	-	expression tag
Dc 20	-	expression tag
Dc 21	-	expression tag
Dg 22	-	expression tag
Dg 23	-	expression tag
Dc 24	-	expression tag
Dg 25	-	expression tag
Da 26	-	expression tag
Dt 27	-	expression tag
Dt 28	-	expression tag
Dt 29	-	expression tag
Dt 30	-	expression tag
Dg 31	-	expression tag
Dg 32	-	expression tag
Dg 33	-	expression tag
Dg 34	-	expression tag
Da 35	-	expression tag
Da 36	-	expression tag
Da 37	-	expression tag
Da 38	-	expression tag
Da 39	-	expression tag
Da 40	-	expression tag
Dt 41	-	expression tag
Da 42	-	expression tag
Da 43	-	expression tag
Dt 44	-	expression tag
Dc 45	-	expression tag
Dg 46	-	expression tag
Dg 47	-	expression tag
Dc 48	-	expression tag
Dc 49	-	expression tag
Dt 50	-	expression tag
Dt 51	-	expression tag
Dg 52	-	expression tag
Da 53	-	expression tag
Dc 54	-	expression tag
Da 55	-	expression tag
Dg 56	-	expression tag
Dc 57	-	expression tag
Dt 58	-	expression tag
Dg 59	-	expression tag
Dc 60	-	expression tag

b, e: 4 - 60 (GenBank: 1190175513)

PDB	External Database	Details
Dt 3	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	30
	Total formula weight	896655.8
Non-Polymers*	Number of molecules	40
	Total formula weight	10469.8
All*	Total formula weight	907125.5

*Water molecules are not included.