8PVB

Structure of GABAAR determined by cryoEM at 100 keV

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Gamma-aminobutyric acid receptor subunit beta-3	polymer	396	45744.3	1	UniProt (P28472) Pfam (PF02931) Pfam (PF02932) In PDB	Homo sapiens (human)	GABA(A) receptor subunit beta-3
2	O	Megabody Mb25	polymer	522	56300.6	1		Lama glama
3	B	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
4	C	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	1	In PDB GlyTouCan (G22768VO)
5	A	HEXADECANE	non-polymer		226.4	1	Chemie (R16)
6	A	DECANE	non-polymer		142.3	4	Chemie (D10)
7	A	HISTAMINE	non-polymer		111.1	1	Chemie (HSM)
8	A	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
9	A	Etomidate	non-polymer		244.3	1	Chemie (V8D)

Sequence modifications

A: 1 - 307 (UniProt: P28472)

PDB	External Database	Details
Glu -54	-	expression tag
Met -53	-	expression tag
Asp -52	-	expression tag
Glu -51	-	expression tag
Lys -50	-	expression tag
Thr -49	-	expression tag
Thr -48	-	expression tag
Gly -47	-	expression tag
Trp -46	-	expression tag
Arg -45	-	expression tag
Gly -44	-	expression tag
Gly -43	-	expression tag
His -42	-	expression tag
Val -41	-	expression tag
Val -40	-	expression tag
Glu -39	-	expression tag
Gly -38	-	expression tag
Leu -37	-	expression tag
Ala -36	-	expression tag
Gly -35	-	expression tag
Glu -34	-	expression tag
Leu -33	-	expression tag
Glu -32	-	expression tag
Gln -31	-	expression tag
Leu -30	-	expression tag
Arg -29	-	expression tag
Ala -28	-	expression tag
Arg -27	-	expression tag
Leu -26	-	expression tag
Glu -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
Pro -22	-	expression tag
Gln -21	-	expression tag
Gly -20	-	expression tag
Gln -19	-	expression tag
Arg -18	-	expression tag
Glu -17	-	expression tag
Pro -16	-	expression tag
Asp -15	-	expression tag
Tyr -14	-	expression tag
Asp -13	-	expression tag
Ile -12	-	expression tag
Pro -11	-	expression tag
Thr -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Thr -1	-	expression tag
Gly 0	-	expression tag
Thr 279	Lys 304	conflict
Ser 308	-	expression tag
Gln 309	-	expression tag
Pro 310	-	expression tag
Ala 311	-	expression tag
Arg 312	-	expression tag
Ala 313	-	expression tag
Ala 314	-	expression tag
Ala 422	-	expression tag
Ile 423	-	expression tag
Asp 424	-	expression tag
Arg 425	-	expression tag
Trp 426	-	expression tag
Ser 427	-	expression tag
Arg 428	-	expression tag
Ile 429	-	expression tag
Val 430	-	expression tag
Phe 431	-	expression tag
Pro 432	-	expression tag
Phe 433	-	expression tag
Thr 434	-	expression tag
Phe 435	-	expression tag
Ser 436	-	expression tag
Leu 437	-	expression tag
Phe 438	-	expression tag
Asn 439	-	expression tag
Leu 440	-	expression tag
Val 441	-	expression tag
Tyr 442	-	expression tag
Trp 443	-	expression tag
Leu 444	-	expression tag
Tyr 445	-	expression tag
Tyr 446	-	expression tag
Val 447	-	expression tag
Asn 448	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	102044.9
Branched	Number of molecules	2
	Total formula weight	1335.2
Non-Polymers*	Number of molecules	9
	Total formula weight	1221.9
All*	Total formula weight	104602.0

*Water molecules are not included.