8PQL

K48-linked ubiquitin chain formation with a cullin-RING E3 ligase and Cdc34: NEDD8-CUL2-RBX1-ELOB/C-FEM1C with trapped UBE2R2-donor UB-acceptor UB-SIL1 peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	C	Ubiquitin-conjugating enzyme E2 R2	polymer	238	27190.9	1	UniProt (Q712K3) Pfam (PF00179) In PDB	Homo sapiens (human)	E2 ubiquitin-conjugating enzyme R2,Ubiquitin carrier protein R2,Ubiquitin-conjugating enzyme E2-CDC34B,Ubiquitin-protein ligase R2
2	E	Polyubiquitin-C,Nucleotide exchange factor SIL1	polymer	704	79226.7	1	UniProt (P0CG48) UniProt (Q9H173) Pfam (PF00240) In PDB	Homo sapiens (human)	BiP-associated protein,BAP
3	H	Protein fem-1 homolog C	polymer	617	68767.3	1	UniProt (Q96JP0) Pfam (PF12796) In PDB	Homo sapiens (human)	FEM1c,FEM1-gamma
4	K	E3 ubiquitin-protein ligase RBX1	polymer	108	12290.0	1	UniProt (P62877) Pfam (PF12678) In PDB	Homo sapiens (human)	E3 ubiquitin-protein transferase RBX1,Protein ZYP,RING finger protein 75,RING-box protein 1,Rbx1,Regulator of cullins 1,ROC1
5	U	Ubiquitin	polymer	685	77120.4	1	UniProt (P0CG48) Pfam (PF00240) In PDB	Homo sapiens (human)
6	A	Cullin-2	polymer	745	87098.9	1	UniProt (Q13617) Pfam (PF00888) Pfam (PF10557) In PDB	Homo sapiens (human)	CUL-2
7	D	Elongin-C	polymer	112	12485.1	1	UniProt (Q15369) Pfam (PF03931) In PDB	Homo sapiens (human)	EloC,Elongin 15 kDa subunit,RNA polymerase II transcription factor SIII subunit C,SIII p15,Transcription elongation factor B polypeptide 1
8	G	Elongin-B	polymer	118	13147.8	1	UniProt (Q15370) Pfam (PF00240) In PDB	Homo sapiens (human)	EloB,Elongin 18 kDa subunit,RNA polymerase II transcription factor SIII subunit B,SIII p18,Transcription elongation factor B polypeptide 2
9	C	5-azanylpentan-2-one	non-polymer		101.1	1	Chemie (SY8)
10	K	ZINC ION	non-polymer		65.4	3	Chemie (ZN)

Sequence modifications

E: 1 - 685 (UniProt: P0CG48)

PDB	External Database	Details
Cys 48	Lys 48	engineered mutation

E: 686 - 704 (UniProt: Q9H173)

PDB	External Database	Details
Pro 698	Ser 455	engineered mutation
Thr 699	Leu 456	engineered mutation
Gln 700	Leu 457	engineered mutation
Gly 701	Lys 458	engineered mutation
Arg 702	Glu 459	engineered mutation
Ala 703	Leu 460	engineered mutation

U: -75 - 609 (UniProt: P0CG48)

PDB	External Database	Details
Cys -28	Lys 48	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	377327.2
Non-Polymers*	Number of molecules	4
	Total formula weight	297.4
All*	Total formula weight	377624.6

*Water molecules are not included.