8PKP
Cryo-EM structure of the apo Anaphase-promoting complex/cyclosome (APC/C) at 3.2 Angstrom resolution
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Anaphase-promoting complex subunit 1 | polymer | 1944 | 216725.5 | 1 | UniProt (Q9H1A4) Pfam (PF12859) Pfam (PF20518) Pfam (PF21282) Pfam (PF18122) | Homo sapiens (human) | APC1,Cyclosome subunit 1,Mitotic checkpoint regulator,Testis-specific gene 24 protein |
| 2 | B (D) | Anaphase-promoting complex subunit 15 | polymer | 121 | 14286.7 | 1 | UniProt (P60006) Pfam (PF15243) | Homo sapiens (human) | APC15 |
| 3 | C, N (G, W) | Anaphase-promoting complex subunit CDC26 | polymer | 85 | 9794.0 | 2 | UniProt (Q8NHZ8) Pfam (PF10471) | Homo sapiens (human) | Anaphase-promoting complex subunit 12,APC12,Cell division cycle protein 26 homolog |
| 4 | D (H) | Anaphase-promoting complex subunit 16 | polymer | 110 | 11678.0 | 1 | UniProt (Q96DE5) Pfam (PF17256) | Homo sapiens (human) | APC16,Cyclosome subunit 16 |
| 5 | E, J (J, P) | Cell division cycle protein 27 homolog | polymer | 824 | 91973.1 | 2 | UniProt (P30260) Pfam (PF12895) Pfam (PF00515) | Homo sapiens (human) | Anaphase-promoting complex subunit 3,APC3,CDC27 homolog,CDC27Hs,H-NUC |
| 6 | F, K (K, Q) | Cell division cycle protein 16 homolog | polymer | 620 | 71747.5 | 2 | UniProt (Q13042) Pfam (PF12895) Pfam (PF13424) | Homo sapiens (human) | Anaphase-promoting complex subunit 6,APC6,CDC16 homolog,CDC16Hs,Cyclosome subunit 6 |
| 7 | G (L) | Anaphase-promoting complex subunit 10 | polymer | 185 | 21282.1 | 1 | UniProt (Q9UM13) Pfam (PF03256) | Homo sapiens (human) | APC10,Cyclosome subunit 10 |
| 8 | H (M) | Anaphase-promoting complex subunit 13 | polymer | 74 | 8528.3 | 1 | UniProt (Q9BS18) Pfam (PF05839) | Homo sapiens (human) | APC13,Cyclosome subunit 13 |
| 9 | I (O) | Anaphase-promoting complex subunit 5 | polymer | 755 | 85179.8 | 1 | UniProt (Q9UJX4) Pfam (PF21371) Pfam (PF12862) | Homo sapiens (human) | APC5,Cyclosome subunit 5 |
| 10 | L, M (U, V) | Cell division cycle protein 23 homolog | polymer | 597 | 68921.0 | 2 | UniProt (Q9UJX2) Pfam (PF04049) | Homo sapiens (human) | Anaphase-promoting complex subunit 8,APC8,Cyclosome subunit 8 |
| 11 | O, P (Y, Z) | Anaphase-promoting complex subunit 7 | polymer | 565 | 63204.0 | 2 | UniProt (Q9UJX3) Pfam (PF13181) Pfam (PF13432) | Homo sapiens (human) | APC7,Cyclosome subunit 7 |
| 12 | Q (I) | Anaphase-promoting complex subunit 4 | polymer | 814 | 93014.1 | 1 | UniProt (Q9UJX5) Pfam (PF12894) Pfam (PF12896) | Homo sapiens (human) | APC4,Cyclosome subunit 4 |
| 13 | R (N) | Anaphase-promoting complex subunit 2 | polymer | 822 | 93939.0 | 1 | UniProt (Q9UJX6) Pfam (PF00888) Pfam (PF08672) | Homo sapiens (human) | APC2,Cyclosome subunit 2 |
| 14 | S (C) | Anaphase-promoting complex subunit 11 | polymer | 84 | 9854.6 | 1 | UniProt (Q9NYG5) Pfam (PF12861) | Homo sapiens (human) | APC11,Cyclosome subunit 11,Hepatocellular carcinoma-associated RING finger protein |
| 15 | T, U, V, W (N, C) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) |
Sequence modifications
I: 1 - 808 (UniProt: Q9UJX5)
| PDB | External Database | Details |
|---|---|---|
| Glu 809 | - | expression tag |
| Asn 810 | - | expression tag |
| Leu 811 | - | expression tag |
| Tyr 812 | - | expression tag |
| Phe 813 | - | expression tag |
| Gln 814 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 19 |
| Total formula weight | 1165767.5 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 261.6 | |
| All* | Total formula weight | 1166029.1 |
